PUBLICATION

Embryonic Fate Map of First Pharyngeal Arch Structures in the sox10: kaede Zebrafish Transgenic Model

Authors
Dougherty, M., Kamel, G., Shubinets, V., Hickey, G., Grimaldi, M., and Liao, E.C.
ID
ZDB-PUB-120907-5
Date
2012
Source
The Journal of craniofacial surgery   23(5): 1333-1337 (Journal)
Registered Authors
Liao, Eric
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Branchial Region/embryology*
  • Embryo, Nonmammalian/metabolism
  • Estradiol/pharmacology
  • Gene Expression Regulation, Developmental
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism
  • In Situ Hybridization
  • Luminescent Proteins/genetics*
  • Luminescent Proteins/metabolism
  • MicroRNAs/genetics
  • MicroRNAs/metabolism
  • Morphogenesis/genetics
  • Neural Crest/embryology
  • SOXE Transcription Factors/genetics
  • SOXE Transcription Factors/metabolism
  • Signal Transduction
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
22948622 Full text @ J. Craniofac. Surg.
Abstract

Cranial neural crest cells follow stereotypic patterns of migration to form craniofacial structures. The zebrafish is a powerful vertebrate genetic model where transgenics with reporter proteins under the transcriptional regulation of lineage-specific promoters can be generated. Numerous studies demonstrate that the zebrafish ethmoid plate is embryologically analogous to the mammalian palate. A fate map correlating embryonic cranial neural crest to defined jaw structures would provide a useful context for the morphogenetic analysis of craniofacial development. To that end, the sox10:kaede transgenic was generated, where sox10 provides lineage restriction to the neural crest. Specific regions of neural crest were labeled at the 10-somite stage by photoconversion of the kaede reporter protein. Lineage analysis was carried out during pharyngeal development in wild-type animals, after miR140 injection, and after estradiol treatment. At the 10-somite stage, cranial neural crest cells anterior of the eye contributed to the median ethmoid plate, whereas cells medial to the eye formed the lateral ethmoid plate and trabeculae and a posterior population formed the mandible. miR-140 overexpression and estradiol inhibition of Hedgehog signaling resulted in cleft development, with failed migration of the anterior cell population to form the median ethmoid plate. The sox10:kaede transgenic line provides a useful tool for neural crest lineage analysis. These studies illustrate the advantages of the zebrafish model for application in morphogenetic studies of vertebrate craniofacial development.

Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes