Nesfatin-1 Regulates the Hypothalamo-Pituitary-Ovarian Axis of Fish
- Authors
- Gonzalez, R., Shepperd, E., Thiruppugazh, V., Lohan, S., Grey, C., Chang, J.P., and Unniappan, S.
- ID
- ZDB-PUB-120817-2
- Date
- 2012
- Source
- Biology of reproduction 87(4): 84 (Journal)
- Registered Authors
- Unniappan, Suraj
- Keywords
- neuroendocrinology, oocyte maturation, pituitary/pituitary hormones, vertebrates, non mammalian, fish, fowl, reptiles, amphibians, zebrafish
- MeSH Terms
-
- Animals
- Calcium-Binding Proteins/genetics
- Calcium-Binding Proteins/metabolism
- Calcium-Binding Proteins/pharmacology
- Calcium-Binding Proteins/physiology*
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- DNA-Binding Proteins/pharmacology
- DNA-Binding Proteins/physiology*
- Female
- Fishes/genetics*
- Fishes/metabolism
- Fishes/physiology
- Follicle Stimulating Hormone/blood
- Follicle Stimulating Hormone/genetics
- Follicle Stimulating Hormone/metabolism
- Gene Expression Regulation/drug effects
- Goldfish/genetics
- Goldfish/metabolism
- Goldfish/physiology
- Gonadotropin-Releasing Hormone/genetics
- Gonadotropin-Releasing Hormone/metabolism
- Hypothalamo-Hypophyseal System/drug effects
- Hypothalamo-Hypophyseal System/metabolism*
- Hypothalamo-Hypophyseal System/physiology
- Hypothalamus/drug effects
- Hypothalamus/metabolism
- Luteinizing Hormone/blood
- Luteinizing Hormone/genetics
- Luteinizing Hormone/metabolism
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Nerve Tissue Proteins/pharmacology
- Nerve Tissue Proteins/physiology*
- Ovary/drug effects
- Ovary/metabolism*
- Ovary/physiology
- Pituitary Gland/drug effects
- Pituitary Gland/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish/physiology
- PubMed
- 22895855 Full text @ Biol. Reprod.
Nesfatin1 is an anorexigen in goldfish. In this study, we provide novel data indicating the presence and regulatory effects of nesfatin1 on the hypothalamopituitaryovarian (HPO) axis of goldfish. NUCB2/Nesfatin1like immunoreactive (ir) cells are present in the hypothalamus, and in the pituitary, suggesting a hypophysiotropic role for nesfatin1. NUCB2/nesfatin-1-like-ir cells colocalize gonadotropin releasing hormone in the nucleus lateralis tuberis posterioris and the nucleus anterior tuberis of the goldfish hypothalamus. The presence of nesfatin-1 with GnRH in these two nuclei implicated in pituitary hormone release suggests a role for nesfatin-1 on gonadotropin secretion. A single intraperitoneal injection of synthetic goldfish nesfatin1 (50 ng/g body weight) resulted in an acute decrease (~75%) in the expression of hypothalamic cGnRH-II and sGnRH mRNAs at 15 minutes post-injection in goldfish. Meanwhile, pituitary LH beta and FSH beta mRNAs were also inhibited (~80%), but only at 60 minutes post-injection. Nesfatin1 administration also resulted in a significant reduction (~60%) in serum LH levels at 60 minutes post-administration. Nesfatin-1 like immunoreactivity was also found in the follicle cells, but not oocytes, in zebrafish and goldfish ovaries. Incubation of zebrafish follicles with nesfatin-1 resulted in a significant reduction in basal germinal vesicle break down (~50%) during the oocyte maturation. In addition, nesfatin-1 also attenuated the stimulatory effects of maturation-inducing hormone on germinal vesicle breakdown. Together, our current results indicate that nesfatin1 is a metabolic hormone with an inhibitory tone on fish reproduction. Nesfatin-1 appears to elicit this suppressive effect through actions on all three tissues in the fish HPO axis.