ZFIN ID: ZDB-PUB-120815-19
Molecular cloning and functional characterization of two duplicated two-cysteine containing type I interferon genes in rock bream Oplegnathus fasciatus
Wan, Q., Wicramaarachchi, W.D., Whang, I., Lim, B.S., Oh, M.J., Jung, S.J., Kim, H.C., Yeo, S.Y., and Lee, J.
Date: 2012
Source: Fish & shellfish immunology   33(4): 886-898 (Journal)
Registered Authors: Yeo, Sang-Yeob
Keywords: type I interferon, rock bream, gene duplication, iridovirus, Mx
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blood Cells/immunology
  • Blood Cells/metabolism
  • Chromosomes, Artificial, Bacterial/genetics
  • Cloning, Molecular
  • Cysteine/genetics
  • Cysteine/immunology*
  • DNA Virus Infections/immunology
  • DNA Virus Infections/veterinary
  • Edwardsiella tarda/physiology
  • Enterobacteriaceae Infections/immunology
  • Enterobacteriaceae Infections/veterinary
  • Escherichia coli/genetics
  • Fish Proteins/genetics
  • Fish Proteins/immunology*
  • GTP-Binding Proteins/chemistry
  • GTP-Binding Proteins/genetics*
  • Gene Duplication
  • Genomic Library
  • Head Kidney/immunology
  • Head Kidney/metabolism
  • Immunity, Innate
  • Interferon Type I/genetics
  • Interferon Type I/immunology*
  • Iridoviridae/physiology
  • Lipopolysaccharides/immunology
  • Lipopolysaccharides/pharmacology
  • Molecular Sequence Data
  • Myxovirus Resistance Proteins
  • Organ Specificity
  • Perciformes/genetics
  • Perciformes/immunology*
  • Phylogeny
  • Poly I-C/immunology
  • Real-Time Polymerase Chain Reaction/veterinary
  • Reverse Transcriptase Polymerase Chain Reaction/veterinary
  • Sequence Alignment/veterinary
  • Sequence Homology, Amino Acid
  • Streptococcal Infections/immunology
  • Streptococcal Infections/veterinary
  • Streptococcus/physiology
PubMed: 22889848 Full text @ Fish Shellfish Immunol.

Two type I interferon (IFN) genes, designated as rbIFN1 and rbIFN2, have been cloned and characterized in rock bream. They are both comprised of 5 exons and 4 introns, and are closely linked on the rock bream chromosome in a unique head-to-head configuration. Both genes encode 183 amino acid (aa) precursor with a putative 17 aa signal peptide in the N-terminal. Only one amino acid divergence is present between two IFNs. Compared with the type I IFNs in higher vertebrates, two rock bream IFNs possess conserved alpha helical structure and share approximately 20% identity in aa sequence. The highest aa sequence homology (83.2%) was found with European seabass IFNs. Phylogenetic analysis grouped two rock bream IFNs into the subgroup-d of two-cysteine containing IFNs. The gene synteny analysis revealed that they are orthologous with the zebrafish IFNφ4 on chromosome-12 and paralogous to each other, which are likely derived from a gene duplication event followed by an inversion. A number of cis-regulatory elements associated with immune response including 15 IRF and 6 NF-κB binding sites are predicted in the shared 4.5 kb 5'-flanking region. Highest constitutive expression of two IFNs was detected in blood cells and skin. Their expression in blood cells and head kidney was up-regulated by lipopolysaccharide, poly I:C, Edwardsiella tarda, Streptococcus iniae and iridovirus. Furthermore, recombinant rbIFN1 protein produced by E. coli induced a rapid and transient expression of the interferon inducible Mx gene in head kidney cells. These results suggest that two duplicated type I IFN genes are involved in rock bream host response to both viral and bacterial pathogens.