ZFIN ID: ZDB-PUB-120727-7
High-Resolution Optical Imaging of Zebrafish Larval Ribbon Synapse Protein RIBEYE, RIM2, and CaV 1.4 by Stimulation Emission Depletion Microscopy
Lv, C., Gould, T.J., Bewersdorf, J., and Zenisek, D.
Date: 2012
Source: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada   18(4): 745-752 (Journal)
Registered Authors: Lv, Caixia, Zenisek, David
Keywords: STED, RIBEYE, zebrafish, retina, synapse, synaptic ribbon, calcium, vesicle
MeSH Terms:
  • Animals
  • Calcium Channels, L-Type/genetics
  • Calcium Channels, L-Type/metabolism*
  • Eye Proteins/genetics
  • Eye Proteins/metabolism*
  • Larva/chemistry
  • Larva/genetics
  • Larva/metabolism
  • Microscopy/methods*
  • Photoreceptor Cells/chemistry
  • Photoreceptor Cells/metabolism
  • Protein Transport
  • Retina/chemistry
  • Retina/embryology
  • Retina/metabolism*
  • Synapses/chemistry
  • Synapses/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 22832038 Full text @ Microsc. Microanal.
ABSTRACT

The synaptic ribbon is a unique presynaptic structure with an intricate morphology in photoreceptors. Because of the resolution limit in conventional fluorescence microscopy, investigating ribbon protein locations has been challenging, especially in the early development stages of model animals. Here, we used stimulated emission depletion microscopy, a super-resolution imaging technique, to look at retina sections in 4 days post-fertilization (dpf) zebrafish. We observed that in photoreceptor cells, RIBEYE and RIM2 are expressed along the synaptic ribbon, with RIM2 consistently located inside of the horseshoe-shaped synaptic ribbon structure with RIBEYE located on the outside. The L-type calcium channel subunit, CACNA1F, exhibited small spot-like staining beneath the RIM2 and RIBEYE structures. Using morpholino antisense oligonucleotides to knock down RIBEYE expression, we observed fewer and shorter ribbons in the photoreceptor outer plexiform layers of 4 dpf fish retina as well as a reduction in RIM2 expression. The clustering of CACNA1F in these blind fish was no longer observed, but instead showed a diffuse expression in the photoreceptor terminal.

ADDITIONAL INFORMATION