ZFIN ID: ZDB-PUB-120727-17
Her4-positive population in the tectum opticum is proliferating neural precursors in the adult zebrafish brain
Jung, S.H., Kim, H.S., Ryu, J.H., Gwak, J.W., Bae, Y.K., Kim, C.H., and Yeo, S.Y.
Date: 2012
Source: Molecules and cells   33(6): 627-632 (Journal)
Registered Authors: Jung, Seung-Hyun, Kim, Hyung-Seok, Yeo, Sang-Yeob
Keywords: adult brain, GRAP, Her4, neural precursor, Notch, transgenic zebrafish
MeSH Terms:
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/metabolism*
  • Brain/cytology
  • Calcium-Binding Proteins/metabolism
  • Cell Proliferation
  • Glial Fibrillary Acidic Protein/metabolism
  • Green Fluorescent Proteins/metabolism
  • Intercellular Signaling Peptides and Proteins/metabolism
  • Membrane Proteins/metabolism
  • Microscopy, Confocal
  • Neural Stem Cells/metabolism*
  • Neural Stem Cells/physiology
  • Receptors, Notch/metabolism
  • Recombinant Fusion Proteins/metabolism
  • Superior Colliculi/cytology*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed: 22570150 Full text @ Mol. Cells

Previous studies have shown that Notch signaling not only regulates the number of early differentiating neurons, but also maintains proliferating neural precursors in the neural tube. Although it is well known that Notch signaling is closely related to the differentiation of adult neural stem cells, none of transgenic zebrafish provides a tool to figure out the relationship between Notch signaling and the differentiation of neural precursors. The goal of this study was to characterize Her4-positive cells by comparing the expression of a fluorescent Her4 reporter in Tg[her4-dRFP] animals with a GFAP reporter in Tg[gfap-GFP] adult zebrafish. BrdU incorporation indicated that dRFP-positive cells were proliferating and a double labeling assay revealed that a significant fraction of the Her4-dRFP positive population was also GFAP-GFP positive. Our observations suggest that a reporter line with Notch-dependent gene expression can provide a tool to examine proliferating neural precursors and/or neuronal/glial precursors in the development of the adult nervous system to examine the model in which Notch signaling maintains proliferating neural precursors in the neural tube.