ZFIN ID: ZDB-PUB-120630-2
Microarray-based gene profiling analysis of müller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish
Qin, Z., and Raymond, P.A.
Date: 2012
Source: Methods in molecular biology (Clifton, N.J.)   884: 255-261 (Chapter)
Registered Authors: Raymond, Pamela
Keywords: none
MeSH Terms:
  • Animals
  • Flow Cytometry
  • Gene Expression Profiling/methods*
  • Light/adverse effects*
  • Neuroglia/metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Retina/cytology
  • Retina/injuries
  • Retina/metabolism*
  • Retina/radiation effects
  • Stem Cells/metabolism*
  • Zebrafish
PubMed: 22688712 Full text @ Meth. Mol. Biol.

Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated Müller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in Müller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS).