Cloning and expression analysis of Fgf5, 6 and 7 during early chick development
- Authors
- Kumar, M., and Chapman, S.C.
- ID
- ZDB-PUB-120531-5
- Date
- 2012
- Source
- Gene expression patterns : GEP 12(7-8): 245-253 (Journal)
- Registered Authors
- Keywords
- FGF/Fgf, Fibroblast Growth Factor, FGFR, FGF receptor, ISH, in situ hybridization
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Chick Embryo
- Chickens/genetics
- Chickens/metabolism*
- Cloning, Molecular
- Conserved Sequence
- Ear, Inner/embryology
- Ear, Inner/metabolism
- Fibroblast Growth Factor 5/genetics*
- Fibroblast Growth Factor 5/metabolism
- Fibroblast Growth Factor 6/genetics*
- Fibroblast Growth Factor 6/metabolism
- Fibroblast Growth Factor 7/genetics*
- Fibroblast Growth Factor 7/metabolism
- Gene Expression Regulation, Developmental*
- In Situ Hybridization
- Likelihood Functions
- Molecular Sequence Data
- Organ Specificity
- Phylogeny
- PubMed
- 22634565 Full text @ Gene Expr. Patterns
FGFs with similar sequences can play different roles depending on the model organisms examined. Determining these roles requires knowledge of spatio-temporal Fgf gene expression patterns. In this study, we report the cloning of chick Fgf5, 6 and 7, and examine their gene expression patterns by whole mount in situ hybridization. We show that Fgf5’s spatio-temporally restricted expression pattern indicates a potentially novel role during inner ear development. Fgf6 and Fgf7, although belonging to different subfamilies with diverged sequences, are expressed in similar patterns within the mesoderm. Alignment of protein sequences and phylogenetic analysis demonstrate that FGF5 and FGF6 are highly conserved between chick, human, mouse and zebrafish. FGF7 is similarly conserved except for the zebrafish, which has considerably diverged.