Live imaging reveals differing roles of macrophages and neutrophils during Zebrafish tail fin regeneration
- Authors
- Li, L., Yan, B., Shi, Y.Q., Zhang, W.Q., and Wen, Z.L.
- ID
- ZDB-PUB-120514-3
- Date
- 2012
- Source
- The Journal of biological chemistry 287(30): 25353-25360 (Journal)
- Registered Authors
- Li, Li, Wen, Zilong
- Keywords
- In vivo imaging, macrophages, neutrophil, regeneration, zebrafish
- MeSH Terms
-
- Animal Fins/cytology*
- Animal Fins/injuries
- Animal Fins/metabolism
- Animals
- Macrophages/cytology*
- Macrophages/metabolism
- Neutrophils/cytology*
- Neutrophils/metabolism
- Regeneration/physiology*
- Tail/cytology*
- Tail/injuries
- Zebrafish/physiology*
- PubMed
- 22573321 Full text @ J. Biol. Chem.
Macrophages and neutrophils are the pivotal immune phagocytes that enter the wound after tissue injury to remove the cell debris and invaded microorganisms, which presumably facilitate the regrowth of injured tissues. Taking advantage of the regeneration abilities of zebrafish and the newly generated myeloid-specific zebrafish lines with labeling of both myeloid lineages, we assessed the behaviors and functions of neutrophils and macrophages during tail fin regeneration. Live imaging showed that within 6 hours post amputation (hpa) - the inflammatory stage, neutrophils were the primary cells scavenging apoptotic bodies and small cell debris, although they had limited phagocytic capacity and quickly underwent apoptosis. From 6 hpa on - the resolution and regeneration stage, macrophages became the dominant scavengers, efficiently resolving inflammation and facilitating tissue remodeling and regrowth. Ablation of macrophages but not neutrophils severely impaired the inflammatory resolution and tissue regeneration, resulting in the formation of large vacuolesin the regenerated fins. In contrast, removal of neutrophils slightly accelerates the regrowth of injured fin. Our study documents the differing behaviors and functions of macrophages and neutrophils during tissue regeneration.