Use of TSHbeta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals
- Authors
- Ji, C., Jin, X., He, J., and Yin, Z.
- ID
- ZDB-PUB-120514-1
- Date
- 2012
- Source
- Toxicology and applied pharmacology 262(2): 149-155 (Journal)
- Registered Authors
- He, Jiangyan, Yin, Zhan
- Keywords
- thyroid-stimulating hormone subunit-β, hypothalamic-pituitary-thyroid, endocrine disrupting, transgenic reporter fish, assessment model
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Endocrine Disruptors/toxicity*
- Green Fluorescent Proteins/genetics*
- Green Fluorescent Proteins/metabolism
- Hypothalamus/drug effects
- Hypothalamus/metabolism
- In Situ Hybridization, Fluorescence
- Models, Animal
- Pituitary Gland/drug effects
- Pituitary Gland/metabolism
- Promoter Regions, Genetic
- Thyroid Gland/drug effects
- Thyroid Gland/metabolism
- Thyrotropin, beta Subunit/genetics*
- Thyrotropin, beta Subunit/metabolism
- Toxicity Tests
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 22571824 Full text @ Tox. App. Pharmacol.
- CTD
- 22571824
Accumulating evidence indicates that a wide range of chemicals have the ability to interfere with the hypothalamic–pituitary–thyroid (HPT) axis. Novel endpoints should be evaluated in addition to existing methods in order to effectively assess the effects of these chemicals on the HPT axis. Thyroid-stimulating hormone subunit β (TSHβ) plays central regulatory roles in the HPT system. We identified the regulatory region that determines the expression level of zebrafish TSHβ in the anterior pituitary. In the transgenic zebrafish with EGFP driven by the TSHβ promoter, the similar responsive patterns between the expression levels of TSHβ:EGFP and endogenous TSHβ mRNA in the pituitary are observed following treatments with goitrogen chemicals and exogenous thyroid hormones (THs). These results suggest that the TSHβ:EGFP transgenic reporter zebrafish may be a useful alternative in vivo model for the assessment of chemicals interfering with the HPT system.