PUBLICATION
3D+t Morphological Processing: Applications to Embryogenesis Image Analysis
- Authors
- Luengo-Oroz, M.A., Pastor, D., Castro, C., Faure, E., Savy, T., Lombardot, B., Rubio-Guivernau, J.L., Duloquin, L., Ledesma-Carbayo, M., Bourgine, P., Peyrieras, N., and Santos, A.
- ID
- ZDB-PUB-120510-8
- Date
- 2012
- Source
- IEEE transactions on image processing : a publication of the IEEE Signal Processing Society 29(9): 792-9 (Journal)
- Registered Authors
- Duloquin, Louise, Savy, Thierry
- Keywords
- 3D+t mathematical morphology, in-vivo light microscopy, spatiotemporal structuring elements, zebrafish
- MeSH Terms
-
- Algorithms
- Animals
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/embryology*
- Embryonic Development/physiology*
- Image Enhancement/methods
- Image Interpretation, Computer-Assisted/methods
- Imaging, Three-Dimensional/methods*
- Microscopy/methods*
- Reproducibility of Results
- Sensitivity and Specificity
- Spatio-Temporal Analysis
- Zebrafish/anatomy & histology*
- Zebrafish/embryology*
- PubMed
- 22562755 Full text @ IEEE Trans. Image Process.
Citation
Luengo-Oroz, M.A., Pastor, D., Castro, C., Faure, E., Savy, T., Lombardot, B., Rubio-Guivernau, J.L., Duloquin, L., Ledesma-Carbayo, M., Bourgine, P., Peyrieras, N., and Santos, A. (2012) 3D+t Morphological Processing: Applications to Embryogenesis Image Analysis. IEEE transactions on image processing : a publication of the IEEE Signal Processing Society. 29(9):792-9.
Abstract
We propose to directly process 3D + t image sequences with mathematical morphology operators using a new classification of the 3D+t structuring elements. Several methods (filtering, tracking, segmentation) dedicated to the analysis of 3D + t datasets of zebrafish embryogenesis are introduced and validated through a synthetic dataset. Then, we illustrate the application of these methods to the analysis of datasets of zebrafish early development acquired with various microscopy techniques. This processing paradigm produces spatio-temporal coherent results as it benefits from the intrinsic redundancy of the temporal dimension and minimizes the needs for human intervention in semi-automatic algorithms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping