ZFIN ID: ZDB-PUB-120416-13
Spectral-Domain Optical Coherence Tomography as a Noninvasive Method to Assess Damaged and Regenerating Adult Zebrafish Retinas
Bailey, T.J., Davis, D.H., Vance, J.E., and Hyde, D.R.
Date: 2012
Source: Investigative ophthalmology & visual science 53(6): 3126-3138 (Journal)
Registered Authors: Bailey, Travis, Hyde, David R.
Keywords: none
MeSH Terms:
  • Animals
  • Dark Adaptation
  • Disease Models, Animal
  • Photoreceptor Cells, Vertebrate/physiology
  • Recovery of Function*
  • Regeneration*
  • Retina/physiology*
  • Retinal Diseases/pathology*
  • Retinal Diseases/physiopathology
  • Tomography, Optical Coherence/methods*
  • Zebrafish
PubMed: 22499984 Full text @ Invest. Ophthalmol. Vis. Sci.

PURPOSE. These experiments assessed the ability of spectral domain optical coherence tomography (SD-OCT) to accurately represent the structural organization of the adult zebrafish retina and reveal the dynamic morphological changes during either light-induced damage and regeneration of photoreceptors or ouabain-induced inner retinal damage. METHODS. Retinas of control dark-adapted adult albino zebrafish were compared to retinas subjected to 24 hours of constant intense light and recovered for up to 8 weeks or ouabain-damaged retinas that recovered for up to 3 weeks. Images were captured and the measurements of retinal morphology were made by the Spectral Domain Optical Coherence Tomography (SD-OCT), and then compared to those obtained by histology of the same eyes. RESULTS. Measurements between SD-OCT and histology were very similar for the undamaged, damaged, and regenerating retinas. Axial measurements of SD-OCT also revealed vitreal morphology that was not readily visualized by histology. CONCLUSIONS. SD-OCT accurately represented retinal lamination and photoreceptor loss and recovery during light-induced damage and subsequent regeneration. SD-OCT was less accurate at detecting the inner nuclear layer in ouabain-damaged retinas, but accurately detected the undamaged outer nuclear layer. Thus, SD-OCT provides a non-invasive and quantitative method to assess the morphology and the extent of damage and repair in the zebrafish retina.