Bailey, T.J., Davis, D.H., Vance, J.E., and Hyde, D.R. (2012) Spectral-Domain Optical Coherence Tomography as a Noninvasive Method to Assess Damaged and Regenerating Adult Zebrafish Retinas. Investigative ophthalmology & visual science. 53(6):3126-3138.
PURPOSE. These experiments assessed the ability of spectral domain optical coherence tomography (SD-OCT) to accurately represent
the structural organization of the adult zebrafish retina and reveal the dynamic morphological changes during either light-induced
damage and regeneration of photoreceptors or ouabain-induced inner retinal damage.
METHODS. Retinas of control dark-adapted adult albino zebrafish were compared to retinas subjected to 24 hours of constant
intense light and recovered for up to 8 weeks or ouabain-damaged retinas that recovered for up to 3 weeks. Images were captured
and the measurements of retinal morphology were made by the Spectral Domain Optical Coherence Tomography (SD-OCT), and then
compared to those obtained by histology of the same eyes.
RESULTS. Measurements between SD-OCT and histology were very similar for the undamaged, damaged, and regenerating retinas.
Axial measurements of SD-OCT also revealed vitreal morphology that was not readily visualized by histology.
CONCLUSIONS. SD-OCT accurately represented retinal lamination and photoreceptor loss and recovery during light-induced damage
and subsequent regeneration. SD-OCT was less accurate at detecting the inner nuclear layer in ouabain-damaged retinas, but
accurately detected the undamaged outer nuclear layer. Thus, SD-OCT provides a non-invasive and quantitative method to assess
the morphology and the extent of damage and repair in the zebrafish retina.