Fluorescent Function-Spacer-Lipid Construct Labelling Allows for Real-Time in Vivo Imaging of Cell Migration and Behaviour in Zebrafish (Danio Rerio)
- Authors
- Lan, C.C., Blake, D., Henry, S., and Love, D.R.
- ID
- ZDB-PUB-120326-27
- Date
- 2012
- Source
- Journal of Fluorescence 22(4): 1055-1063 (Journal)
- Registered Authors
- Love, Donald R.
- Keywords
- kodecyte, labeling, fluorescence, zebrafish
- MeSH Terms
-
- Animals
- Blood Cells/cytology
- Cell Movement*
- Fluorescein/chemistry
- Fluorescein/metabolism
- Fluorescent Dyes/chemistry*
- Fluorescent Dyes/metabolism*
- Lipids/chemistry*
- Microscopy, Fluorescence
- Molecular Imaging/methods*
- Neovascularization, Physiologic
- Time Factors
- Transplants
- Zebrafish*/physiology
- PubMed
- 22434405 Full text @ J. Fluoresc.
Real-time in vivo imaging of cell migration and behavior has advanced our understanding of physiological processes in situ, especially in the field of immunology. We carried out the transplantation of a mixed population of blood cells from adult zebrafish (Danio rerio) to 2 day old embryos. The blood cells were treated ex vivo with Function-Spacer-Lipid constructs (FSL) incorporating either fluorescein or Atto488 fluorophores (FSL-FLRO4-I or -II). Excellent labeling efficiency was demonstrated by epifluorescence microscopy and FACScan analysis. Real-time video imaging of the recipient fish showed that the functionality of these cells was retained and not affected by the labeling. The usefulness of FSL-FLRO4-I as a contrast agent in microangiography was explored. Overall, we found both FSL-FLRO4-I and-II promising labeling dyes for real-time in vivo imaging in zebrafish.