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ZFIN ID: ZDB-PUB-120227-9
2-O-sulfotransferase regulates Wnt signaling, cell adhesion and cell cycle during zebrafish epiboly
Cadwalader, E.L., Condic, M.L., and Yost, H.J.
Date: 2012
Source: Development (Cambridge, England)   139(7): 1296-1305 (Journal)
Registered Authors: Yost, H. Joseph
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cadherins/metabolism
  • Cell Adhesion
  • Cell Cycle
  • Cytoskeletal Proteins/metabolism
  • Gene Expression Regulation, Developmental
  • Glycogen Synthase Kinase 3/metabolism
  • Heparan Sulfate Proteoglycans/chemistry
  • Ligands
  • Models, Biological
  • Signal Transduction
  • Sulfotransferases/chemistry*
  • Sulfotransferases/physiology
  • Transcription, Genetic
  • Wnt Proteins/metabolism*
  • Zebrafish
  • Zebrafish Proteins/chemistry*
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology
  • beta Catenin/metabolism
PubMed: 22357927 Full text @ Development

O-sulfotransferases modify heparan sulfate proteoglycans (HSPGs) by catalyzing the transfer of a sulfate to a specific position on heparan sulfate glycosaminoglycan (GAG) chains. Although the roles of specific HSPG modifications have been described in cell culture and invertebrates, little is known about their functions or abilities to modulate specific cell signaling pathways in vertebrate development. Here, we report that 2-O-sulfotransferase (2-OST) is an essential component of canonical Wnt signaling in zebrafish development. 2-OST-defecient embryos have reduced GAG chain sulfation and are refractory to exogenous Wnt8 overexpression. Embryos in which maternally encoded 2-OST is knocked down have normal activation of several zygotic mesoderm, endoderm and ectoderm patterning genes, but have decreased deep cell adhesion and fail to initiate epiboly, which can be rescued by re-expression of 2-OST protein. Reduced cell adhesion and altered cell cycle regulation in 2-OST-deficient embryos are associated with decreased β-catenin and E-cadherin protein levels at cell junctions, and these defects can be rescued by reactivation of the intracellular Wnt pathway, utilizing stabilized β-catenin or dominant-negative Gsk3, but not by overexpression of Wnt8 ligand. Together, these results indicate that 2-OST functions within the Wnt pathway, downstream of Wnt ligand signaling and upstream of Gsk3β and β-catenin intracellular localization and function.