PUBLICATION

High-Contrast Reversible Fluorescence Photoswitching of Dye-Crosslinked Dendritic Nanoclusters in Living Vertebrates

Authors
Kim, Y., Jung, H.Y., Choe, Y.H., Lee, C., Ko, S.K., Koun, S., Choi, Y., Chung, B.H., Park, B.C., Huh, T.L., Shin, I., and Kim, E.
ID
ZDB-PUB-120207-15
Date
2012
Source
Angewandte Chemie (International ed. in English)   51(12): 2878-2882 (Journal)
Registered Authors
Huh, Tae-Lin, Koun, Soonil
Keywords
dendrimer, fluorescence, FRET, photoswitch, zebrafish
MeSH Terms
  • Animals
  • Carbocyanines/chemistry
  • Dendrimers/chemistry*
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes/chemistry*
  • HeLa Cells
  • Humans
  • Microscopy, Fluorescence
  • Nanoparticles/chemistry*
  • Ultraviolet Rays
  • Zebrafish
PubMed
22307986 Full text @ Angew. Chem. Int. Ed. Engl.
Abstract

Dendrimers crosslinked with a photochromic diarylethene (blue in picture) were labeled with the fluorescent dye Cy3 (red) for reversible fluorescence photoswitching. If the obtained nanoclusters are irradiated with UV light, the Cy3 fluorescence is quenched by the diarylethene in its ring-closed form, whereas upon irradiation with visible light, the nanoclusters show fluorescence. High-contrast fluorescence imaging was achieved inside a living zebrafish.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes