ZFIN ID: ZDB-PUB-120203-1
Highly selective cysteine detection and bioimaging in zebrafish through emission color change of water-soluble conjugated polymer-based assay complex
Kwon, N.Y., Kim, D., Jang, G., Lee, J.H., So, J.H., Kim, C.H., Kim, T.H., and Lee, T.S.
Date: 2012
Source: ACS applied materials & interfaces   4(3): 1429-1433 (Journal)
Registered Authors: So, Ju-Hoon
Keywords: none
MeSH Terms:
  • Animals
  • Biological Assay/methods*
  • Color
  • Cysteine/metabolism*
  • Imaging, Three-Dimensional/methods*
  • Microscopy, Phase-Contrast
  • Polymerization
  • Polymers/chemistry*
  • Solubility
  • Spectrometry, Fluorescence
  • Water/chemistry*
  • Zebrafish/metabolism*
PubMed: 22296511 Full text @ ACS Appl. Mater. Interfaces

A new concept for rapid, label-free cysteine sensing method is proposed via possible naked eye-detection of red-to-blue emission color change. Intermolecular exciton migration in conjugated polyelectrolyte-based assay complex is adopted to enhance selectivity and sensitivity for cysteine sensing by formation and dissociation of polymer-Hg2+-thymine assay complex in the absence and presence of cysteine, respectively. The assay complex shows red emission due to cooperative aggregation of conjugated polyelectrolyte, thymine, and Hg2+. Upon exposure to cysteine, the assay complex dissociates into individual molecules showing transparent, blue-emitting solution, because cysteine extracts Hg2+ from the assay complex via more favorable binding between cysteine and Hg2+.