PUBLICATION

Molecular Response to Ultraviolet Radiation Exposure in Fish Embryos: Implications for Survival and Morphological Development

Authors
Torres-Nuñez, E., Sobrino, C., Neale, P.J., Ceinos, R.M., Du, S.J., and Rotllant, J.
ID
ZDB-PUB-120117-15
Date
2012
Source
Photochemistry and photobiology   88(3): 701-707 (Journal)
Registered Authors
Du, Shao Jun (Jim), Rotllant, Josep
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • DNA Primers
  • DNA Repair
  • Genes, p53
  • Larva/growth & development
  • Osteonectin/genetics
  • Polymerase Chain Reaction
  • Ultraviolet Rays*
  • Zebrafish/embryology*
  • Zebrafish/growth & development
PubMed
22242699 Full text @ Photochem. Photobiol.
Abstract

UVR exposure is known to cause developmental defects in a variety of organisms including aquatic species but little is known about the underlying molecular mechanisms. In this work we used zebrafish (Danio rerio) embryos as a model system to characterize the UVR effects on fish species. Larval viability was measured for embryos exposed to several UVR spectral treatments by using a solar simulator lamp and an array of UV cut-off filters under controlled conditions in the laboratory. Survival rate and occurrence of development abnormalities, mainly caudal (posterior) notochord bending/torsion, were seriously affected in UV exposed larvae reaching values of 53% and 72% respectively, compared to non-UV exposed larvae after 6 days post fertilization (dpf). In order to elucidate the molecular mechanisms involved, a matricellular glycoprotein named osteonectin and the expression of a DNA-repair related gene, p53, were studied in relation to UVR exposure. The results indicate that osteonectin and p53 expression were increased under UVR exposure due to wavelengths shorter than 335 nm (i.e. mainly UVB) and 350 nm (i.e. short UVA and UVB), respectively. Furthermore, parallel experiments with microinjections of osteonectin capped-RNA showed that malformations induced by osteonectin overexpression were similar to those observed after a UVR exposure. Consequently this study shows a potential role of osteonectin in morphological deformities induced by solar UV radiation in zebrafish embryos.

Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping