ZFIN ID: ZDB-PUB-120111-9
Evolutionary history of c-myc in teleosts and characterization of the duplicated c-myca genes in goldfish embryos
Marandel, L., Labbe, C., Bobe, J., and Le Bail, P.Y.
Date: 2012
Source: Molecular reproduction and development   72(2): 85-96 (Journal)
Registered Authors: Bobe, Julien
Keywords: none
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian
  • Evolution, Molecular*
  • Fishes/embryology
  • Fishes/genetics*
  • Gene Duplication*/physiology
  • Gene Expression Regulation, Developmental
  • Genes, myc/genetics*
  • Genome
  • Goldfish/embryology
  • Goldfish/genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Synteny
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed: 22213278 Full text @ Mol. Reprod. Dev.
ABSTRACT

c-Myc plays an important role during embryogenesis in mammals, but little is known about its function during embryonic development in teleosts. In addition, the evolutionary history of c-myc gene in teleosts remains unclear, and depending on the species, a variable number of gene duplicates exist in teleosts. To gain new insight into c-myc genes in teleosts, the present study was designed to clarify the evolutionary history of c-myc gene(s) in teleosts and to subsequently characterize DNA methylation and early embryonic expression patterns in a cyprinid fish. Our results show that a duplication of c-myc gene occurred before or around the teleost radiation, as a result of the teleost-specific whole genome duplication giving rise to c-myca and c-mycb in teleosts and was followed by a loss of the c-mycb gene in the Gasterosteiforms and Tetraodontiforms. Our data also demonstrate that both c-myc genes previously identified in carp and goldfish are co-orthologs of the zebrafish c-myca. These results indicate the presence of additional c-myca duplication in Cyprininae. We were able to identify differences between the expression patterns of the two goldfish c-myca genes in oocytes and early embryos. These differences suggest a partial sub-functionalization of c-myca genes after duplication. Despite differences in transcription patterns, both of the c-myca genes displayed similar DNA methylation patterns during early development and in gametes. Together, our results clarify the evolutionary history of the c-myc gene in teleosts and provide new insight into the involvement of c-myc in early embryonic development in cyprinids.

ADDITIONAL INFORMATION