header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-PUB-120111-3
Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish
Hu, S.N., Yu, H., Zhang, Y.B., Wu, Z.L., Yan, Y.C., Li, Y.X., Li, Y.Y., and Li, Y.P.
Date: 2012
Source: FEBS letters 586(3): 222-228 (Journal)
Registered Authors:
Keywords: sox31, MBT, apoptosis
Microarrays: GEO:GSE32914
MeSH Terms:
  • Alternative Splicing/drug effects
  • Animals
  • Apoptosis/drug effects
  • Apoptosis/genetics*
  • Blastula/cytology*
  • Blastula/drug effects
  • Blastula/metabolism*
  • Female
  • Gene Expression Regulation, Developmental/drug effects
  • Gene Expression Regulation, Developmental/genetics
  • Morpholinos/pharmacology
  • Mothers
  • RNA Splicing/drug effects*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • SOX Transcription Factors/genetics*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics*
  • Zygote/cytology
  • Zygote/drug effects*
  • Zygote/metabolism
PubMed: 22209980 Full text @ FEBS Lett.
FIGURES
ABSTRACT
Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microarray analysis of mRNAs collected at the dome stage revealed that the Sb MO impairs activation of a large set of zygotic genes and reduces degradation of maternal mRNA during MBT. Furthermore, an apoptotic response occurs in Sb morphants at about 6 hpf. SoxB1 family genes including sox31 thus play an essential role for early embryos traversing the transitional stage.
ADDITIONAL INFORMATION