PUBLICATION

Visualizing vascular networks in zebrafish: an introduction to microangiography

Authors
Schmitt, C.E., Holland, M.B., and Jin, S.W.
ID
ZDB-PUB-120111-22
Date
2012
Source
Methods in molecular biology (Clifton, N.J.)   843: 59-67 (Chapter)
Registered Authors
Jin, Suk-Won
Keywords
microangiography, zebrafish, vascular lumen, cardiovascular development
MeSH Terms
  • Angiography/instrumentation
  • Angiography/methods*
  • Animals
  • Blood Vessels/anatomy & histology
  • Blood Vessels/embryology*
  • Microinjections
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
PubMed
22222521 Full text @ Meth. Mol. Biol.
Abstract
Visualizing the circulatory pattern in developing embryos becomes an essential technique for the field of cardiovascular biology. In the zebrafish model system, there are currently several techniques available to visualize the circulatory pattern. Microangiography is a simple technique in which a fluorescent dye is injected directly into the Sinus Venosus and/or the Posterior Cardinal Vein, allowing for the rapid labeling and easy detection of patent vessels. Here, we compare microangiography to other vascular labeling techniques, describe the benefits and potential applications of microangiography, and give step by step instructions for microangiography.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes