PUBLICATION
Alterations of protein profile in zebrafish liver cells exposed to methyl parathion: A membrane proteomics approach
- Authors
- Huang, Q., and Huang, H.Q.
- ID
- ZDB-PUB-120105-86
- Date
- 2012
- Source
- Chemosphere 87(1): 68-76 (Journal)
- Registered Authors
- Keywords
- methyl parathion, ZFL cell line, proteomics, membrane protein, biomarkers
- MeSH Terms
-
- Animals
- Cell Line
- Gene Expression/drug effects
- Insecticides/toxicity*
- Liver/cytology
- Liver/drug effects*
- Liver/metabolism
- Methyl Parathion/toxicity*
- Proteome/genetics
- Proteome/metabolism*
- Proteomics
- RNA, Messenger/metabolism
- Water Pollutants, Chemical/toxicity
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 22182705 Full text @ Chemosphere
- CTD
- 22182705
Citation
Huang, Q., and Huang, H.Q. (2012) Alterations of protein profile in zebrafish liver cells exposed to methyl parathion: A membrane proteomics approach. Chemosphere. 87(1):68-76.
Abstract
Methyl parathion (MP) is an extensively used organophosphorus pesticide, which has been associated with a wide spectrum of toxic effects on environmental organisms. The aim of this study is to investigate the alterations of membrane protein profiles in zebrafish liver (ZFL) cell line exposed to MP for 24 h using proteomic approaches. Two-dimensional gel electrophoresis revealed a total of 13 protein spots, whose expression levels were significantly altered by MP. These differential proteins were subjected to matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry analysis, and nine proteins were identified to be membrane proteins, among which seven were up-regulated, while two were down-regulated. In addition, the mRNA levels corresponding to these differential membrane proteins were further analyzed by quantitative real-time PCR. And the differential expression of arginase-2 was specially validated via Western blotting. Regarding the physiological functions, these proteins are involved in molecular chaperon, cytoskeleton system, cell metabolism, signal transduction, transport and hormone receptor respectively, suggesting the complexity of MP-mediated toxicity to ZFL cell. These data could provide useful insights for better understanding the hepatotoxic mechanisms of MP and develop novel protein biomarkers for effectively monitoring MP contamination level in aquatic environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping