Characterization of a Weak Allele of Zebrafish cloche Mutant

Ma, N., Huang, Z., Chen, X., He, F., Wang, K., Liu, W., Zhao, L., Xu, X., Liao, W., Ruan, H., Luo, S., and Zhang, W.
PLoS One   6(11): e27540 (Journal)
Registered Authors
Chen, Xiaohui, Huang, Zhibin, Liu, Wei, Ma, Ning, Ruan, Hua, Wang, Kun, Zhao, Lingfeng
Embryos, Hematopoiesis, Bone marrow cells, Zebrafish, Mesoderm, Hematopoietic stem cells, Endothelial cells, In situ hybridization
MeSH Terms
  • Alleles*
  • Animals
  • Blood Vessels/embryology
  • Blood Vessels/metabolism
  • Cell Lineage/genetics
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Erythropoiesis/genetics
  • Gene Expression Regulation, Developmental
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/metabolism
  • In Situ Hybridization
  • Mutation/genetics*
  • Myelopoiesis/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
22132109 Full text @ PLoS One

Hematopoiesis is a complicated and dynamic process about which the molecular mechanisms remain poorly understood. Danio rerio (zebrafish) is an excellent vertebrate system for studying hematopoiesis and developmental mechanisms. In the previous study, we isolated and identified a cloche172 (clo172) mutant, a novel allele compared to the original cloche (clo) mutant, through using complementation test and initial mapping. Here, according to whole mount in-situ hybridization, we report that the endothelial cells in clo172 mutant embryos, although initially developed, failed to form the functional vascular system eventually. In addition, further characterization indicates that the clo172 mutant exhibited weaker defects instead of completely lost in primitive erythroid cells and definitive hematopoietic cells compared with the clos5 mutant. In contrast, primitive myeloid cells were totally lost in clo172 mutant. Furthermore, these reappeared definitive myeloid cells were demonstrated to initiate from the remaining hematopoietic stem cells (HSCs) in clo172 mutant, confirmed by the dramatic decrease of lyc in clo172runx1w84x double mutant. Collectively, the clo172 mutant is a weak allele compared to the clos5 mutant, therefore providing a model for studying the early development of hematopoietic and vascular system, as well as an opportunity to further understand the function of the cloche gene.

Genes / Markers
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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes