Anti-tumor and anti-angiogenic effects of Macrothelypteris viridifrons and its constituents by HPLC-DAD/MS analysis
- Authors
- Wei, A., Zhou, D., Ruan, J., Cai, Y., Xiong, C., and Wu, G.
- ID
- ZDB-PUB-111130-12
- Date
- 2012
- Source
- Journal of ethnopharmacology 139(2): 373-80 (Journal)
- Registered Authors
- Keywords
- Macrothelypteris viridifrons, flavonoids, anti-tumor, anti-angiogenic, zebrafish model
- MeSH Terms
-
- Angiogenesis Inhibitors/chemistry
- Angiogenesis Inhibitors/isolation & purification
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Animals, Genetically Modified
- Antigens, CD34/metabolism
- Antineoplastic Agents, Phytogenic/chemistry
- Antineoplastic Agents, Phytogenic/isolation & purification
- Antineoplastic Agents, Phytogenic/pharmacology*
- Carcinoma, Hepatocellular/drug therapy*
- Carcinoma, Hepatocellular/metabolism
- Carcinoma, Hepatocellular/pathology
- Cell Line, Tumor
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Chromatography, High Pressure Liquid*
- Dose-Response Relationship, Drug
- Ferns*/chemistry
- Flavonoids/analysis
- Human Umbilical Vein Endothelial Cells/drug effects
- Liver Neoplasms/drug therapy*
- Liver Neoplasms/metabolism
- Liver Neoplasms/pathology
- Male
- Mass Spectrometry*
- Mice
- Neovascularization, Physiologic/drug effects*
- Plants, Medicinal
- Tumor Burden/drug effects
- Vascular Endothelial Growth Factor A/metabolism
- Vascular Endothelial Growth Factor Receptor-2/genetics
- Vascular Endothelial Growth Factor Receptor-2/metabolism
- Zebrafish/genetics
- PubMed
- 22123201 Full text @ J. Ethnopharmacol.
Ethnopharmacological relevance
Macrothelypteris viridifrons is widely distributed in south of China and has been used as folk medicine to treat cancer, hydropsy, and traumatic bleeding.
Aim of the study
To investigate the chemical constituents and the anti-tumor and anti-angiogenic effects of Macrothelypteris viridifrons.
Materials and methods
An HPLC–DAD/MS technique was used to determine the flavonoid profile of Macrothelypteris viridifrons. The anti-tumor effect of Macrothelypteris viridifrons was evaluated by in vivo mice bearing H22 hepatoma cells transplantation tumor model. And the anti-angiogenic activity was investigated by measuring the effects on the in vitro proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs). Furthermore, the in vivo zebrafish model was applied to evaluate the anti-angiogenic effect of Macrothelypteris viridifrons.
Results
18 flavonoids were identified from Macrothelypteris viridifrons. Administration of Macrothelypteris viridifrons significantly inhibited the tumor growth and the expression of vascular endothelial growth factor (VEGF) and CD34. Meanwhile, Macrothelypteris viridifrons showed significant inhibition on proliferation, migration and tube formation of HUVECs in vitro and the intersegmental vessels formation in zebrafish model.
Conclusions
Macrothelypteris viridifrons showed significant anti-tumor and anti-angiogenic effects and might be developed as a novel anti-tumor drug.