ZFIN ID: ZDB-PUB-111129-21
Neurodevelopmental low-dose bisphenol A exposure leads to early life-stage hyperactivity and learning deficits in adult zebrafish
Saili, K.S., Corvi, M.M., Weber, D.N., Patel, A.U., Das, S.R., Przybyla, J., Anderson, K.A., and Tanguay, R.L.
Date: 2012
Source: Toxicology 291(1-3): 83-92 (Journal)
Registered Authors: Tanguay, Robert L., Weber, Dan
Keywords: Bisphenol A, zebrafish, behavior, learning, hyperactivity, endocrine disruptor
MeSH Terms:
  • Animals
  • Behavior, Animal/drug effects
  • Benzhydryl Compounds
  • Chromatography, High Pressure Liquid
  • Dose-Response Relationship, Drug
  • Embryo, Nonmammalian/metabolism
  • Endocrine Disruptors/pharmacokinetics
  • Endocrine Disruptors/toxicity*
  • Environmental Pollutants/pharmacokinetics
  • Environmental Pollutants/toxicity*
  • Estradiol/pharmacology
  • Hydrazines/pharmacology
  • Hyperkinesis/chemically induced*
  • Larva
  • Learning Disorders/chemically induced*
  • Learning Disorders/psychology*
  • Maze Learning/drug effects
  • Phenols/pharmacokinetics
  • Phenols/toxicity*
  • Receptors, Estrogen/drug effects
  • Receptors, G-Protein-Coupled/drug effects
  • Reversal Learning/drug effects
  • Teratogens/toxicity
  • Zebrafish/physiology*
PubMed: 22108044 Full text @ Toxicology

Developmental bisphenol A (BPA) exposure has been implicated in adverse behavior and learning deficits. The mode of action underlying these effects is unclear. The zebrafish model was employed to investigate the neurobehavioral effects of developmental bisphenol A (BPA) exposure. The objectives of this study were to identify whether low-dose, developmental BPA exposure affects larval zebrafish locomotor behavior and whether learning deficits occur in adults exposed during development. Two control compounds, 17β-estradiol (an estrogen receptor ligand) and GSK4716 (a synthetic estrogen-related receptor gamma ligand), were included. Larval toxicity assays were used to determine appropriate BPA, 17β-estradiol, and GSK4716 concentrations for behavior testing. BPA tissue uptake was analyzed using HPLC and lower doses were extrapolated using a linear regression analysis. Larval behavior tests were conducted using a ViewPoint Zebrabox. Adult learning tests were conducted using a custom-built T-maze. BPA exposure to d30 μM was non-teratogenic in zebrafish. Neurodevelopmental BPA exposure to 0.01, 0.1, or 1 μM led to larval hyperactivity or learning deficits in adult zebrafish. Exposure to 0.1 μM 17β-estradiol or GSK4716 also led to larval hyperactivity. This study demonstrates the efficacy of using the larval zebrafish model for studying the neurobehavioral effects of low-dose developmental BPA exposure.