Regulation of intrahepatic biliary duct morphogenesis by Claudin 15-like b

Cheung, I.D., Bagnat, M., Ma, T.P., Datta, A., Evason, K., Moore, J.C., Lawson, N.D., Mostov, K.E., Moens, C.B., and Stainier, D.Y.
Developmental Biology   361(1): 68-78 (Journal)
Registered Authors
Bagnat, Michel, Cheung, Isla, Evason, Kimberley, Lawson, Nathan, Ma, Taylur, Moens, Cecilia, Moore, John, Stainier, Didier
claudin, liverdevelopment, zebrafish, biliary duct remodeling, biliary cells, biliary duct morphogenesis, tight junctions, cholestasis
MeSH Terms
  • Animals
  • Bile Ducts, Intrahepatic/cytology
  • Bile Ducts, Intrahepatic/growth & development*
  • Bile Ducts, Intrahepatic/metabolism
  • Cell Line
  • Cell Polarity/physiology
  • Claudins/genetics
  • Claudins/metabolism*
  • Dogs
  • Epithelial Cells/metabolism
  • Fluorescent Antibody Technique
  • Hepatocytes/metabolism*
  • In Situ Hybridization
  • Larva/growth & development
  • Larva/metabolism
  • Microscopy, Electron, Transmission
  • Morphogenesis/physiology*
  • Mutation/genetics
  • Tight Junctions/metabolism*
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
22020048 Full text @ Dev. Biol.
The intrahepatic biliary ducts transport bile produced by the hepatocytes out of the liver. Defects in biliary cell differentiation and biliary duct remodeling cause a variety of congenital diseases including Alagille Syndrome and polycystic liver disease. While the molecular pathways regulating biliary cell differentiation have received increasing attention (Lemaigre, 2010), less is known about the cellular behavior underlying biliary duct remodeling. Here, we have identified a novel gene, claudin 15-like b (cldn15lb), which exhibits a unique and dynamic expression pattern in the hepatocytes and biliary epithelial cells in zebrafish. Claudins are tight junction proteins that have been implicated in maintaining epithelial polarity, regulating paracellular transport, and providing barrier function. In zebrafish cldn15lb mutant livers, tight junctions are observed between hepatocytes, but these cells show polarization defects as well as canalicular malformations. Furthermore, cldn15lb mutants show abnormalities in biliary duct morphogenesis whereby biliary epithelial cells remain clustered together and form a disorganized network. Our data suggest that Cldn15lb plays an important role in the remodeling process during biliary duct morphogenesis. Thus, cldn15lb mutants provide a novel in vivo model to study the role of tight junction proteins in the remodeling of the biliary network and hereditary cholestasis.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes