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ZFIN ID: ZDB-PUB-111027-57
The Methyltransferases PRMT4/CARM1 and PRMT5 Control Differentially Myogenesis in Zebrafish
Batut, J., DuboƩ, C., and Vandel, L.
Date: 2011
Source: PLoS One 6(10): e25427 (Journal)
Registered Authors: Batut, Julie, Vandel, Laurence
Keywords: none
MeSH Terms:
  • Animals
  • Gene Expression Regulation, Developmental
  • Kinetics
  • Muscle Development*
  • Protein-Arginine N-Methyltransferases/genetics
  • Protein-Arginine N-Methyltransferases/metabolism*
  • Zebrafish/growth & development*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 22016767 Full text @ PLoS One
FIGURES
ABSTRACT

In vertebrates, skeletal myogenesis involves the sequential activation of myogenic factors to lead ultimately to the differentiation into slow and fast muscle fibers. How transcriptional co-regulators such as arginine methyltransferases PRMT4/CARM1 and PRMT5 control myogenesis in vivo remains poorly understood. Loss-of-function experiments using morpholinos against PRMT4/CARM1 and PRMT5 combined with in situ hybridization, quantitative polymerase chain reaction, as well as immunohistochemistry indicate a positive, but differential, role of these enzymes during myogenesis in vivo. While PRMT5 regulates myod, myf5 and myogenin expression and thereby slow and fast fiber formation, PRMT4/CARM1 regulates myogenin expression, fast fiber formation and does not affect slow fiber formation. However, our results show that PRMT4/CARM1 is required for proper slow myosin heavy chain localization. Altogether, our results reveal a combinatorial role of PRMT4/CARM1 and PRMT5 for proper myogenesis in zebrafish.

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