Vitamin E Deficiency Decreases Long-Chain PUFA in Zebrafish (Danio rerio)
- Authors
- Lebold, K.M., Jump, D.B., Miller, G.W., Wright, C.L., Labut, E.M., Barton, C.L., Tanguay, R.L., and Traber, M.G.
- ID
- ZDB-PUB-111027-45
- Date
- 2011
- Source
- The Journal of nutrition 141(12): 2113-8 (Journal)
- Registered Authors
- Tanguay, Robyn L.
- Keywords
- none
- MeSH Terms
-
- Animals
- Arachidonic Acid/administration & dosage
- Diet*
- Docosahexaenoic Acids/analysis
- Fatty Acids, Unsaturated/analysis*
- Fatty Acids, Unsaturated/metabolism*
- Female
- Gene Expression Regulation/drug effects
- Linoleic Acid/administration & dosage
- Linoleic Acid/analysis
- Lipid Peroxidation
- Male
- RNA/isolation & purification
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Vitamin E Deficiency/metabolism*
- Zebrafish
- alpha-Linolenic Acid/administration & dosage
- alpha-Linolenic Acid/analysis
- alpha-Tocopherol/administration & dosage
- PubMed
- 22013196 Full text @ J. Nutr.
α-Tocopherol is a required, lipid-soluble antioxidant that protects PUFA. We hypothesized that α-tocopherol deficiency in zebrafish compromises PUFA status. Zebrafish were fed for 1 y either an α-tocopherol-sufficient (E+; 500 mg α-tocopherol/kg) or -deficient (E-; 1.1 mg α-tocopherol/kg) diet containing α-linolenic (ALA) and linoleic (LA) acids but without arachidonic acid (ARA), EPA, or DHA. Vitamin E deficiency in zebrafish decreased by ~20% (n-6) (P < 0.05) and (n-3) (P < 0.05) PUFA and increased the (n-6):(n-3) PUFA ratio (P < 0.05). In E- compared to E+ females, long chain-PUFA status was impaired, as assessed by a ~60% lower DHA:ALA ratio (P < 0.05) and a ~50% lower ARA:LA ratio (P < 0.05). fads2 (P < 0.05) and elovl2 (P < 0.05) mRNA expression was doubled in E- compared to E+ fish. Thus, inadequate vitamin E status led to a depletion of PUFA that may be a result of either or both increased lipid peroxidation and an impaired ability to synthesize sufficient PUFA, especially (n-3) PUFA.