PUBLICATION

Neurohypophyseal hormones manipulation modulate social and anxiety-related behavior in zebrafish

Authors

Braida, D., Donzelli, A., Martucci, R., Capurro, V., Busnelli, M., Chini, B., and Sala, M.

ID

ZDB-PUB-111013-14

Date

2012

Source

Psychopharmacology 220(2): 319-330 (Journal)

Registered Authors

Keywords

oxytocin, vasopressin, isotocin, vasotocin, shoaling, anxiety, social behavior, selective antagonist

MeSH Terms

Animals
Anti-Anxiety Agents/pharmacology*
Antidiuretic Hormone Receptor Antagonists
Dose-Response Relationship, Drug
Fear/drug effects*
Indoles/pharmacology
Ornipressin/analogs & derivatives
Ornipressin/pharmacology
Oxytocin/analogs & derivatives
Oxytocin/antagonists & inhibitors
Oxytocin/pharmacology
Oxytocin/physiology*
Pituitary Hormones, Posterior/pharmacology
Pituitary Hormones, Posterior/physiology
Pyrrolidines/pharmacology
Radioligand Assay/methods
Radioligand Assay/statistics & numerical data
Receptors, Oxytocin/antagonists & inhibitors
Receptors, Oxytocin/physiology
Receptors, Vasopressin/physiology*
Social Behavior*
Swimming
Vasopressins/antagonists & inhibitors
Vasopressins/pharmacology
Vasopressins/physiology*
Vasotocin/antagonists & inhibitors
Vasotocin/pharmacology
Vasotocin/physiology*
Zebrafish

PubMed

21956239 Full text @ Psychpharma

Abstract

Rationale:

Oxytocin (OT) and arginine-vasopressin (AVP) regulate social behavior in mammals. Zebrafish (Danio rerio) allows higher throughput and ease in studying human brain disorders.

Objectives:

This study investigated in zebrafish the effect of non-mammalian homologs isotocin (IT) and vasotocin (AVT) in comparison with OT/AVP on social behavior and fear response to predator. The mechanism was studied using the most human selective OT and AVP receptor antagonists.

Methods

Zebrafish were injected i.m. with increasing doses (0.001–40 ng/kg) of the neuropeptides. DesGly-NH₂-d(CH₂)₅-[d-Tyr²,Thr⁴]OVT) for OT receptor, SR 49059 for V1a subtype receptor, and SSR-149415 for V1b subtype receptor were injected i.m. 10 min before each agonist.

Results:

All the peptides increased social preference and reduced fear to predator response in a dose-dependent manner interpolated by symmetrical parabolas. AVT/AVP were more potent to elicit anxiolytic than social effect while IT and OT were equally potent. All the antagonists dose-dependently inhibited both the effects induced by the neuropeptides. The ratio between the ED50 obtained for blocking the OT-induced effects on social preference and fear response to predator was very high only for desglyDTTyrOVT (160). SR49059 showed the highest ratio in blocking AVP-induced effects (807). The less selective antagonist appeared to be SSR149415.

Conclusions:

For the first time, IT/AVT and OT/AVP were found to modulate in zebrafish, social behavior, unrelated to sex, and fear to predator response through at least two different receptors. Zebrafish is confirmed as a valid, reliable model to study deficit in social behavior characteristic of some psychiatric disorders.

Genes / Markers

Figures

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Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

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Orthology

Engineered Foreign Genes

Mapping