PUBLICATION
Targeted mutagenesis of zebrafish: Use of zinc finger nucleases
- Authors
- Leong, I.U., Lai, D., Lan, C.C., Johnson, R., Love, D.R., Johnson, R., and Love, D.R.
- ID
- ZDB-PUB-110921-48
- Date
- 2011
- Source
- Birth defects research. Part C, Embryo today : reviews 93(3): 249-255 (Review)
- Registered Authors
- Love, Donald R.
- Keywords
- zebrafish, zinc finger nucleases, mutagenesis
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Deoxyribonucleases/metabolism*
- Gene Targeting*
- Humans
- Mutagenesis*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zinc Fingers/genetics*
- PubMed
- 21932433 Full text @ Birth Defects Res. C Embryo Today
Citation
Leong, I.U., Lai, D., Lan, C.C., Johnson, R., Love, D.R., Johnson, R., and Love, D.R. (2011) Targeted mutagenesis of zebrafish: Use of zinc finger nucleases. Birth defects research. Part C, Embryo today : reviews. 93(3):249-255.
Abstract
The modeling of human disease in the zebrafish (Danio rerio) is moving away from chemical mutagensis and transient downregulation using morpholino oligomers to more targeted and stable transgenic methods. In this respect, zinc finger nucleases offer a means of introducing mutations at targeted sites at high efficiency. We describe here the development of zinc finger nucleases and their general use in model systems with a focus on the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping