ZFIN ID: ZDB-PUB-110921-4
Tol2-mediated Transgenesis, Gene Trapping, Enhancer Trapping, and the Gal4-UAS System
Abe, G., Suster, M.L., and Kawakami, K.
Date: 2011
Source: Methods in cell biology 104: 23-49 (Chapter)
Registered Authors: Kawakami, Koichi, Suster, Maximiliano
Keywords: enhancer, gene trapping, germline, microinjection, transgenesis, transposable
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Blotting, Southern/methods
  • Chromosomes, Artificial, Bacterial
  • Cloning, Molecular/methods*
  • DNA Transposable Elements*
  • Genes, Reporter
  • Green Fluorescent Proteins/biosynthesis
  • Green Fluorescent Proteins/genetics
  • Microinjections/methods
  • Mutagenesis, Insertional/methods*
  • Recombination, Genetic
  • Transfection/methods
  • Zebrafish/genetics*
PubMed: 21924155 Full text @ Meth. Cell. Biol.
The Tol2 transposable element was originally found in the genome of the Japanese medaka fish (Oryzias latipes). Tol2 contains a gene encoding an active transposase that can catalyze DNA transposition in vertebrate cells. In zebrafish, Tol2 generates genomic integrations in the germ cells very efficiently. By using the Tol2 transposition system, we have developed important genetic methods including transgenesis, gene trapping, enhancer trapping, and the Gal4-UAS system in zebrafish. In this chapter, we describe how these methods can be performed.