PUBLICATION
Possible role of serotoninergic system in the neurobehavioral impairment induced by acute methylmercury exposure in zebrafish (Danio rerio)
- Authors
- Maximino, C., Araújo, J., Leão, L.K., Grisolia, A.B., Oliveira, K.R., Lima, M.G., Batista, E.D., Crespo-López, M.E., Gouveia, A., and Herculano, A.M.
- ID
- ZDB-PUB-110901-23
- Date
- 2011
- Source
- Neurotoxicology and teratology 33(6): 727-34 (Journal)
- Registered Authors
- Lima, Monica Gomes, Maximino, Caio
- Keywords
- zebrafish, oxidative stress, anxiety, methylmercury, serotonin
- MeSH Terms
-
- Animals
- Anxiety/chemically induced
- Anxiety/metabolism
- Anxiety/physiopathology
- Behavior, Animal/drug effects*
- Brain/drug effects*
- Brain/metabolism
- Chromatography, High Pressure Liquid
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Female
- Lipid Peroxidation/drug effects
- Male
- Mercury Poisoning, Nervous System/physiopathology*
- Methylmercury Compounds/toxicity*
- Oxidative Stress/drug effects
- Serotonin/metabolism*
- Subcellular Fractions/metabolism
- Zebrafish/metabolism*
- Zebrafish/physiology
- PubMed
- 21871955 Full text @ Neurotoxicol. Teratol.
Citation
Maximino, C., Araújo, J., Leão, L.K., Grisolia, A.B., Oliveira, K.R., Lima, M.G., Batista, E.D., Crespo-López, M.E., Gouveia, A., and Herculano, A.M. (2011) Possible role of serotoninergic system in the neurobehavioral impairment induced by acute methylmercury exposure in zebrafish (Danio rerio). Neurotoxicology and teratology. 33(6):727-34.
Abstract
Adult zebrafish were treated acutely with methylmercury (1.0 or 5.0 μg g- 1, i.p.) and, 24 h after treatment, were tested in two behavioral models of anxiety, the novel tank and the light/dark preference tests. At the smaller dose, methylmercury produced a marked anxiogenic profile in both tests, while the greater dose produced hyperlocomotion in the novel tank test. These effects were accompanied by a decrease in extracellular levels of serotonin, and an increase in extracellular levels of tryptamine-4,5-dione, a partially oxidized metabolite of serotonin. A marked increase in the formation of malondialdehyde, a marker of oxidative stress, accompanied these parameters. It is suggested that methylmercury-induced oxidative stress produced mitochondrial dysfunction and originated tryptamine-4,5-dione, which could have further inhibited tryptophan hydroxylase. These results underscore the importance of assessing acute, low-level neurobehavioral effects of methylmercury.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping