PUBLICATION
Zebrafish obesogenic test: a tool for screening molecules that target adiposity
- Authors
- Tingaud-Sequeira, A., Ouadah, N., and Babin, P.J.
- ID
- ZDB-PUB-110713-10
- Date
- 2011
- Source
- Journal of Lipid Research 52(9): 1765-1772 (Journal)
- Registered Authors
- Babin, Patrick J.
- Keywords
- adipocytes, adipose tissue, diet and dietary lipids, fluorescence microscopy, lipids, lipolysis and fatty acid metabolism, obesity, toxicology, alternative testing assay, obesogen
- MeSH Terms
-
- Adipose Tissue, White/cytology
- Adipose Tissue, White/drug effects
- Adipose Tissue, White/metabolism
- Adiposity*
- Animals
- Biological Assay/methods*
- Diet
- Environmental Pollutants/adverse effects
- Fluorescent Dyes/metabolism
- Humans
- Larva/anatomy & histology
- Larva/drug effects
- Larva/metabolism
- Models, Animal*
- Obesity*
- Oxazines/metabolism
- Trialkyltin Compounds/pharmacology
- Triglycerides/metabolism
- Zebrafish*/anatomy & histology
- Zebrafish*/growth & development
- Zebrafish*/metabolism
- PubMed
- 21724975 Full text @ J. Lipid Res.
Citation
Tingaud-Sequeira, A., Ouadah, N., and Babin, P.J. (2011) Zebrafish obesogenic test: a tool for screening molecules that target adiposity. Journal of Lipid Research. 52(9):1765-1772.
Abstract
Dietary and xenobiotic compounds may alter endocrine signaling and lipid homeostasis, thus inducing obesity. We describe a short-term assay method, the zebrafish obesogenic (ZO) test, for examining the effects of diet, drugs, and environmental contaminants, singly or in combination, on white adipose tissue dynamics in live larvae. The ZO test is an intermediate step in obesity research, between in vitro and rodent assays, and may be also used to study the effect of environmental toxicants on the adiposity of aquatic species. The procedure, using Nile Red fluorescent probe to reveal adipocyte lipid droplets, is suitable for pharmaceutical or toxicological screening. Larvae treated at an environmentally-relevant concentration of tributyltin chloride, an environmental obesogen, exhibited a remarkable increase in adiposity, irrespective of the lipid composition of the background diet. Exogenous compounds, e.g. rosiglitazone or tributyltin chloride, known to increase adiposity in the fasting state, were classified as obesogenic. Anti-obesogenic compounds favored a decrease in adiposity in the fasting state. The ZO test, using adipocyte lipid droplet size and adiposity as its endpoints, is a whole-organism alternative testing assay for obesogenic and anti-obesogenic compounds and mixtures and provides relevant information for environmental and human risk assessments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping