PUBLICATION
Recording the adult zebrafish cerebral field potential during pentylenetetrazole seizures
- Authors
- Pineda, R., Beattie, C.E., and Hall, C.W.
- ID
- ZDB-PUB-110629-18
- Date
- 2011
- Source
- Journal of Neuroscience Methods 200(1): 20-8 (Journal)
- Registered Authors
- Beattie, Christine, Pineda, Ricardo
- Keywords
- Epilepsy, zebrafish, EEG, pentylenetetrazole, seizure, amplifier
- MeSH Terms
-
- Action Potentials/drug effects
- Action Potentials/physiology*
- Amplifiers, Electronic*
- Animals
- Behavior, Animal/drug effects
- Behavior, Animal/physiology
- Cerebrum/drug effects
- Cerebrum/physiopathology*
- Convulsants/pharmacology
- Disease Models, Animal
- Electroencephalography/instrumentation
- Electroencephalography/methods*
- Electronics, Medical/instrumentation
- Electronics, Medical/methods
- Epilepsy/chemically induced
- Epilepsy/physiopathology*
- Pentylenetetrazole/pharmacology
- Zebrafish/physiology*
- PubMed
- 21689682 Full text @ J. Neurosci. Methods
Citation
Pineda, R., Beattie, C.E., and Hall, C.W. (2011) Recording the adult zebrafish cerebral field potential during pentylenetetrazole seizures. Journal of Neuroscience Methods. 200(1):20-8.
Abstract
Although the zebrafish is increasingly used as a model organism to study epilepsy, no standard electrophysiological technique for recording electrographic seizures in adult fish exists. The purpose of this paper is to introduce a readily implementable technique for recording pentylenetetrazole seizures in the adult zebrafish. We find that we can consistently record a high quality field potential over the zebrafish cerebrum using an amplification of 5000V/V and bandpass filtering at corner frequencies of 1.6 and 16Hz. The cerebral field potential recordings show consistent features in the baseline, pre-seizure, seizure and post-seizure time periods that can be easily recognized by visual inspection as is the case with human and rodent electroencephalogram. Furthermore, numerical analysis of the field potential at the time of seizure onset reveals an increase in the total power, bandwidth and peak frequency in the power spectrum, as is also the case with human and rodent electroencephalogram. The techniques presented herein stand to advance the utility of the adult zebrafish in the study of epilepsy by affording an equivalent to the electroencephalogram used in mammalian models and human patients.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping