|ZFIN ID: ZDB-PUB-110613-25|
tcf21+ epicardial cells adopt non-myocardial fates during zebrafish heart development and regeneration
Kikuchi, K., Gupta, V., Wang, J., Holdway, J.E., Wills, A.A., Fang, Y., and Poss, K.D.
|Source:||Development (Cambridge, England) 138(14): 2895-902 (Journal)|
|Registered Authors:||Fang, Yi, Gupta, Vikas, Holdway, Jennifer, Kikuchi, Kazu, Poss, Kenneth D., Wang, Jinhu, Wills, Airon|
|Keywords:||heart regeneration, heart development, cardiomyocyte, epicardium, zebrafish, genetic fate-mapping|
|PubMed:||21653610 Full text @ Development|
Kikuchi, K., Gupta, V., Wang, J., Holdway, J.E., Wills, A.A., Fang, Y., and Poss, K.D. (2011) tcf21+ epicardial cells adopt non-myocardial fates during zebrafish heart development and regeneration. Development (Cambridge, England). 138(14):2895-902.
ABSTRACTRecent lineage-tracing studies have produced conflicting results about whether the epicardium is a source of cardiac muscle cells during heart development. Here, we examined the developmental potential of epicardial tissue in zebrafish during both embryonic development and injury-induced heart regeneration. We found that upstream sequences of the transcription factor gene tcf21 activated robust, epicardium-specific expression throughout development and regeneration. Cre recombinase-based, genetic fate-mapping of larval or adult tcf21+ cells revealed contributions to perivascular cells, but not cardiomyocytes, during each form of cardiogenesis. Our findings indicate that natural epicardial fates are limited to non-myocardial cell types in zebrafish.