PUBLICATION
tcf21+ epicardial cells adopt non-myocardial fates during zebrafish heart development and regeneration
- Authors
- Kikuchi, K., Gupta, V., Wang, J., Holdway, J.E., Wills, A.A., Fang, Y., and Poss, K.D.
- ID
- ZDB-PUB-110613-25
- Date
- 2011
- Source
- Development (Cambridge, England) 138(14): 2895-902 (Journal)
- Registered Authors
- Fang, Yi, Gupta, Vikas, Holdway, Jennifer, Kikuchi, Kazu, Poss, Kenneth D., Wang, Jinhu, Wills, Airon
- Keywords
- heart regeneration, heart development, cardiomyocyte, epicardium, zebrafish, genetic fate-mapping
- MeSH Terms
-
- Regeneration/genetics
- Regeneration/physiology*
- Cell Differentiation/physiology
- Cell Lineage/physiology
- Morphogenesis/genetics
- Morphogenesis/physiology*
- Fluorescent Antibody Technique
- Zebrafish Proteins/metabolism*
- Animals
- Zebrafish/embryology*
- Transcription Factors/metabolism*
- Pericardium/cytology*
- Pericardium/metabolism
- Gene Expression Regulation, Developmental/physiology*
- Heart/embryology*
- Animals, Genetically Modified
- PubMed
- 21653610 Full text @ Development
Citation
Kikuchi, K., Gupta, V., Wang, J., Holdway, J.E., Wills, A.A., Fang, Y., and Poss, K.D. (2011) tcf21+ epicardial cells adopt non-myocardial fates during zebrafish heart development and regeneration. Development (Cambridge, England). 138(14):2895-902.
Abstract
Recent lineage-tracing studies have produced conflicting results about whether the epicardium is a source of cardiac muscle
cells during heart development. Here, we examined the developmental potential of epicardial tissue in zebrafish during both
embryonic development and injury-induced heart regeneration. We found that upstream sequences of the transcription factor
gene tcf21 activated robust, epicardium-specific expression throughout development and regeneration. Cre recombinase-based, genetic
fate-mapping of larval or adult tcf21+ cells revealed contributions to perivascular cells, but not cardiomyocytes, during each form of cardiogenesis. Our findings
indicate that natural epicardial fates are limited to non-myocardial cell types in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping