|ZFIN ID: ZDB-PUB-110609-39|
Live imaging of endogenous Collapsin response mediator protein-1 expression at subcellular resolution during zebrafish nervous system development
Jayasena, C.S., and Bronner-Fraser, M.
|Source:||Gene expression patterns : GEP 11(7): 395-400 (Journal)|
|Registered Authors:||Bronner-Fraser, Marianne, Jayasena, Chaturani (Saku)|
|Keywords:||Crmp1; Zebrafish; Nervous system; Development|
|PubMed:||21628002 Full text @ Gene Expr. Patterns|
Jayasena, C.S., and Bronner-Fraser, M. (2011) Live imaging of endogenous Collapsin response mediator protein-1 expression at subcellular resolution during zebrafish nervous system development. Gene expression patterns : GEP. 11(7):395-400.
ABSTRACTCollapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins that are functionally important during vertebrate development. We have generated a zebrafish gene trap line that produces fluorescently tagged Crmp1 protein, which can be dynamically tracked in living fish at subcellular resolution. The results show that Crmp1 is expressed in numerous sites in the developing nervous system. Early expression is apparent in the forebrain, epiphysis, optic tectum and the developing spinal cord. In the larval brain, Crmp1 is expressed in several distinct brain regions, such as the telencephalon, habenula and cerebellum. In addition, it is expressed in the spinal cord in a manner that persists in the larva. The results suggest that this Crmp1 protein trap line offers a powerful tool to track selected neuronal populations at high resolution.