PUBLICATION
Regulation of Zebrafish Hatching by Tetraspanin cd63
- Authors
- Trikić, M.Z., Monk, P., Roehl, H., and Partridge, L.J.
- ID
- ZDB-PUB-110609-30
- Date
- 2011
- Source
- PLoS One 6(5): e19683 (Journal)
- Registered Authors
- Roehl, Henry
- Keywords
- Embryos, Zebrafish, Lysosomes, Morpholino, Cats, Fertilization, Polymerase chain reaction, Reverse transcriptase-polymerase chain reaction
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/physiology*
- Models, Animal
- Molecular Sequence Data
- Tetraspanin 30/metabolism*
- Zebrafish/physiology*
- PubMed
- 21625559 Full text @ PLoS One
Citation
Trikić, M.Z., Monk, P., Roehl, H., and Partridge, L.J. (2011) Regulation of Zebrafish Hatching by Tetraspanin cd63. PLoS One. 6(5):e19683.
Abstract
Tetraspanins cause the clustering of membrane proteins into a level of organisation essential for cellular function. Given the importance and complicated nature of this mechanism, we attempted a novel approach to identify the function of a single component in a biologically relevant context. A morpholino knockdown strategy was used to investigate the role of cd63, a membrane protein associated with intracellular transport and a melanoma marker, in embryonic zebrafish. By using three separate morpholinos targeting cd63, we were able to identify a specific phenotype. Strikingly, morphant fish failed to hatch due to the lack of secreted proteolytic enzymes required for chorion-softening. The morphology of the hatching gland at both the cellular and intracellular levels was disorganised, suggesting a role for cd63 in the functioning of this organ. This work identifies a specific role for cd63 in the zebrafish embryo and provides evidence for the suitability of zebrafish as a model system for the investigation of tetraspanin enriched microdomains.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping