PUBLICATION

Fgf20b is required for the ectomesenchymal fate establishment of cranial neural crest cells in zebrafish

Authors
Yamauchi, H., Goto, M., Katayama, M., Miyake, A., and Itoh, N.
ID
ZDB-PUB-110609-14
Date
2011
Source
Biochemical and Biophysical Research Communications   409(4): 705-10 (Journal)
Registered Authors
Itoh, Nobuyuki, Miyake, Ayumi
Keywords
Fgf; Fgf20b; Cranial neural crest; Pharyngeal arch; Ectomesenchyme; Zebrafish
MeSH Terms
  • Animals
  • Cell Lineage
  • Ectoderm/cytology*
  • Ectoderm/metabolism
  • Elastic Cartilage/embryology
  • Elastic Cartilage/metabolism
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism*
  • Gene Knockdown Techniques
  • Mesoderm/cytology*
  • Mesoderm/metabolism
  • Neural Crest/cytology*
  • Neural Crest/metabolism
  • Pharynx/embryology
  • Pharynx/metabolism
  • Skull/embryology*
  • Skull/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
21621510 Full text @ Biochem. Biophys. Res. Commun.
Abstract
In cranial skeletal development, the establishment of the ectomesenchymal lineage within the cranial neural crest is of great significance. Fgfs are polypeptide growth factors with diverse functions in development and metabolism. Fgf20b knockdown zebrafish embryos showed dysplastic neurocranial and pharyngeal cartilages. Ectomesenchymal cells from cranial neural crest cells were significantly decreased in Fgf20b knockdown embryos, but cranial neural crest cells with a non-ectomesnchymal fate were increased. However, the proliferation and apoptosis of cranial neural crest cells were essentially unchanged. Fgfr1 knockdown embryos also showed dysplastic neurocranial and pharyngeal cartilages. The present findings indicate that Fgf20b is required for ectomesenchymal fate establishment via the activation of Fgfr1 in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes