ZFIN ID: ZDB-PUB-110603-7
Molecular characterization of the autophagy-related gene Beclin-1 from the olive flounder (Paralichthys olivaceus)
Kong, H.J., Moon, J.Y., Nam, B.H., Kim, Y.O., Kim, W.J., Lee, J.H., Kim, K.K., Kim, B.S., Yeo, S.Y., Lee, C.H., Kang, K.I., and Lee, S.J.
Date: 2011
Source: Fish & shellfish immunology   31(2): 189-95 (Journal)
Registered Authors: Kong, Hee Jeong, Yeo, Sang-Yeob
Keywords: beclin-1, autophagy-related gene, paralichthys olivaceous, viral hemorrhagic septicemia virus (VHSV), innate immunity
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Apoptosis Regulatory Proteins/chemistry
  • Apoptosis Regulatory Proteins/genetics
  • Apoptosis Regulatory Proteins/metabolism*
  • Autophagy
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary/genetics
  • DNA, Complementary/immunology
  • Fish Proteins/chemistry
  • Fish Proteins/genetics
  • Fish Proteins/metabolism*
  • Flounder/genetics*
  • Flounder/immunology*
  • Flounder/metabolism
  • Flounder/virology
  • Gene Expression Regulation
  • Molecular Sequence Data
  • Novirhabdovirus*
  • Phylogeny
  • RNA, Messenger/genetics
  • Rhabdoviridae Infections/immunology
  • Rhabdoviridae Infections/veterinary
  • Rhabdoviridae Infections/virology
  • Sequence Alignment
PubMed: 21605687 Full text @ Fish Shellfish Immunol.
Autophagy is an important cellular response to starvation and stress, and plays critical roles in embryogenesis, development, cell death, cancer, and immunity. Beclin-1 is one of the central regulators of autophagy in mammals. In the present study, we isolated a PoBeclin-1 cDNA from the olive flounder (Paralichthys olivaceus) by screening a flounder gill cDNA library and rapid amplification of cDNA ends (RACE). The PoBeclin-1 cDNA we isolated encodes a 447-amino acid polypeptide containing a conserved Bcl-2-binding domain. The deduced amino acid sequence of PoBeclin-1 showed high degrees of sequence identity (80.5–95.3%) with Beclin-1 from human, frog, mouse, zebrafish, and pufferfish. PoBeclin-1 transcripts were detected from 1 day post-hatching and were found to be ubiquitously expressed in the healthy flounder. Expression of PoBeclin-1 mRNA was increased in the kidney and spleen of flounders challenged with viral hemorrhagic septicemia virus (VHSV). When infected with VHSV, PoBeclin-1-overexpressing HINAE cells had low level (about 26%) of VHSV G transcripts compared to control cells. Taken together, these results suggest that PoBeclin-1 may play a role in the innate immune response to viral infection in the flounder.