PUBLICATION
Ontogeny of globin expression in zebrafish (Danio rerio)
- Authors
- Tiedke, J., Gerlach, F., Mitz, S.A., Hankeln, T., and Burmester, T.
- ID
- ZDB-PUB-110603-23
- Date
- 2011
- Source
- Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 181(8): 1011-21 (Journal)
- Registered Authors
- Keywords
- cytoglobin, danio rerio, embryogenesis, hemoglobin, myoglobin, neuroglobin, oxygen, zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Embryonic Development/genetics
- Gene Expression/genetics*
- Globins/genetics*
- Hemoglobins/genetics
- Larva/metabolism
- Myoglobin/genetics
- Nerve Tissue Proteins/genetics
- Oocytes/metabolism
- Ovum/metabolism
- Phylogeny
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology*
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics*
- Zygote/metabolism
- PubMed
- 21614507 Full text @ J. Comp. Physiol. B
Citation
Tiedke, J., Gerlach, F., Mitz, S.A., Hankeln, T., and Burmester, T. (2011) Ontogeny of globin expression in zebrafish (Danio rerio). Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology. 181(8):1011-21.
Abstract
Respiratory proteins are responsible for transport and storage of oxygen. It is well established that specific requirements
for oxygen during vertebrate ontogeny cause switches of hemoglobin chain expression. Here, we characterize the developmental
profiles of zebrafish (Danio rerio) globins by means of quantitative real-time reverse transcription PCR. The total mRNA levels of the hemoglobin chains, including
a newly identified embryonic α-chain, as well as myoglobin, neuroglobin, cytoglobin 1 and 2, and globin X were estimated.
mRNAs of all globins were detectable in unfertilized eggs, suggesting maternal storage. Embryonic α- and β-hemoglobin mRNA
peaked at hatching and the switch to adult hemoglobin expression occurred 16 dpf. Enhanced myoglobin mRNA levels were detected
~31 h post-fertilization (hpf), coinciding with the heart and the muscle development, while neuroglobin mRNA expression pattern
correlates with the formation of the nervous system. Amounts of myoglobin and neuroglobin mRNA were similar within an order
of magnitude throughout the ontogeny, tentatively supporting a respiratory role of neuroglobin. Cytoglobin 2 mRNA levels increased
gradually, whereas cytoglobin 1 mRNA levels increased strongly after ~31 hpf, which is in agreement with a function in cell
proliferation. Globin X mRNA level was highest in oocytes, but low in later stages. Together, these data suggest a specific
role for each globin, which are also associated with certain events in fish development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping