header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-PUB-110524-33
The Apoptotic Regulator Nrz Controls Cytoskeletal Dynamics via the Regulation of Ca(2)+ Trafficking in the Zebrafish Blastula
Popgeorgiev, N., Bonneau, B., Ferri, K.F., Prudent, J., Thibaut, J., and Gillet, G.
Date: 2011
Source: Developmental Cell   20(5): 663-676 (Journal)
Registered Authors: Gillet, Germain
Keywords: none
MeSH Terms:
  • Animals
  • Apoptosis
  • Blastula/cytology
  • Blastula/metabolism*
  • Calcium/metabolism*
  • Cytoskeleton/metabolism*
  • Endoplasmic Reticulum/metabolism
  • HeLa Cells
  • Humans
  • Proto-Oncogene Proteins/metabolism*
  • Zebrafish
  • Zebrafish Proteins/metabolism*
PubMed: 21571223 Full text @ Dev. Cell
FIGURES
ABSTRACT
Bcl-2 family members are key regulators of apoptosis. Their involvement in other cellular processes has been so far overlooked. We have studied the role of the Bcl-2 homolog Nrz in the developing zebrafish. Nrz was found to be localized to the yolk syncytial layer, a region containing numerous mitochondria and ER membranes. Nrz knockdown resulted in developmental arrest before gastrulation, due to free Ca(2+) increase in the yolk cell, activating myosin light chain kinase, which led to premature contraction of actin-myosin cables in the margin and separation of the blastomeres from the yolk cell. In the yolk syncytial layer, Nrz appears to prevent the release of Ca(2+) from the endoplasmic reticulum by directly interacting with the IP3R1 Ca(2+) channel. Thus, the Bcl-2 family may participate in early development, not only by controlling apoptosis but also by acting on cytoskeletal dynamics and cell movements via Ca(2+) fluxes inside the embryo.
ADDITIONAL INFORMATION