PUBLICATION
Mechanisms underlying the protective effect of zinc and selenium against cadmium-induced oxidative stress in zebrafish Danio rerio
- Authors
- Banni, M., Chouchene, L., Said, K., Kerkeni, A., and Messaoudi, I.
- ID
- ZDB-PUB-110519-20
- Date
- 2011
- Source
- Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 24(6): 981-92 (Journal)
- Registered Authors
- Keywords
- oxidative stress, gene expression, Dc, Zn, Se, zebrafish, protective effect
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Cadmium/toxicity*
- Catalase/genetics
- Catalase/metabolism
- Female
- Gene Expression/drug effects
- Glutathione Peroxidase/genetics
- Glutathione Peroxidase/metabolism
- Humans
- Lipid Peroxidation
- Liver/physiology
- Metallothionein/genetics
- Metallothionein/metabolism
- Ovary/physiology
- Oxidative Stress/drug effects*
- RNA, Messenger/metabolism
- Selenium/metabolism*
- Superoxide Dismutase/genetics
- Superoxide Dismutase/metabolism
- Zebrafish/metabolism*
- Zinc/metabolism*
- PubMed
- 21533903 Full text @ Biometals
Citation
Banni, M., Chouchene, L., Said, K., Kerkeni, A., and Messaoudi, I. (2011) Mechanisms underlying the protective effect of zinc and selenium against cadmium-induced oxidative stress in zebrafish Danio rerio. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. 24(6):981-92.
Abstract
The present study was designed to elucidate the protective effect mechanism of Zinc (Zn) and Selenium (Se) on cadmium (Cd)-induced oxidative stress in zebrafish. For this purpose we investigate the response of oxidative stress markers, metallothionein accumulation and gene expression in liver and ovary of female zebrafish exposed to 0,4 mg/l Cd in water and supplemented with Zn (5 mg kg(-1)) and/or Se (2 mg kg(-1)) for 21 days in their diet. Liver and ovary Cd uptake was evaluated after the exposure period. Cd exposure significantly inhibited the antioxidant enzyme activities termed as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxydase (GPx) and caused a pronounced malondialdehyde (MDA) accumulation in both organs. Co-administration of Zn and Se reversed the Cd-induced toxicity in liver and ovary measured as MDA accumulation. Interestingly, gene expression patterns of Cat, CuZnSod and Gpx were up-regulated when related enzymatic activities were altered. Zebrafish metallothionein transcripts (zMt) significantly decreased in tissues of fish supplemented with Zn and/or Se when compared to Cd-exposed fish. Our data would suggest that Zn and Se protective mechanism against Cd-induced oxidative stress is more depending on the correction of the proteins biological activities rather than on the transcriptional level of related genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping