PUBLICATION
Somatic cells derived from haploid larvae are feasible as donors for nuclear transplant in zebrafish. Preliminary results
- Authors
- Cardona-Costa, J., Pérez-Camps, M., and García-Ximénez, F.
- ID
- ZDB-PUB-110426-9
- Date
- 2012
- Source
- Zygote (Cambridge, England) 20(3): 277-280 (Journal)
- Registered Authors
- Cardona-Costa, Jose
- Keywords
- Cell culture, haploid, nuclear transplant, verebrate, zebrafish
- MeSH Terms
-
- Animals
- Cell Nucleus/metabolism
- Cloning, Organism
- Haploidy*
- Larva/growth & development*
- Nuclear Transfer Techniques
- Oocytes/metabolism
- Zebrafish/embryology
- Zebrafish/metabolism*
- PubMed
- 21429287 Full text @ Zygote
Citation
Cardona-Costa, J., Pérez-Camps, M., and García-Ximénez, F. (2012) Somatic cells derived from haploid larvae are feasible as donors for nuclear transplant in zebrafish. Preliminary results. Zygote (Cambridge, England). 20(3):277-280.
Abstract
Summary Somatic cells derived from zebrafish haploid larval (both androgenetic and gynogenetic) cultures were used as donors for nuclear transplant into non-enucleated oocytes. Nuclei were transplanted either before or simultaneously with oocyte activation in the central region and in the incipient animal pole, respectively. Against expected results, 20% of transplanted embryos during oocyte activation using cells of gynogenetic origin reached the 100% epiboly stage, even two survived for up to 5 days, whereas no development was observed when cells from androgenetic origin were used. Results derived from this work open a novel possibility of studying somatic cell reprogramming and imprinting phenomena in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping