PUBLICATION
A highly conserved cis-regulatory motif directs differential gonadal synexpression of Dmrt1 transcripts during gonad development
- Authors
- Herpin, A., Nakamura, S., Wagner, T.U., Tanaka, M., and Schartl, M.
- ID
- ZDB-PUB-110413-4
- Date
- 2009
- Source
- Nucleic acids research 37(5): 1510-1520 (Journal)
- Registered Authors
- Schartl, Manfred, Tanaka, Minoru, Wagner, Toni
- Keywords
- none
- MeSH Terms
-
- 3' Untranslated Regions/chemistry*
- Animals
- Cells, Cultured
- Fish Proteins/biosynthesis
- Fish Proteins/genetics*
- Gene Expression Regulation, Developmental*
- Gonads/embryology
- Gonads/growth & development
- Gonads/metabolism*
- Humans
- Mesoderm/metabolism
- Oryzias/embryology
- Oryzias/genetics
- Oryzias/growth & development
- Protein Biosynthesis
- RNA Stability
- RNA, Messenger/metabolism
- RNA-Binding Proteins/metabolism
- Regulatory Sequences, Ribonucleic Acid*
- Transcription Factors/biosynthesis
- Transcription Factors/genetics*
- PubMed
- 19139075 Full text @ Nucleic Acids Res.
Citation
Herpin, A., Nakamura, S., Wagner, T.U., Tanaka, M., and Schartl, M. (2009) A highly conserved cis-regulatory motif directs differential gonadal synexpression of Dmrt1 transcripts during gonad development. Nucleic acids research. 37(5):1510-1520.
Abstract
Differential gene expression largely accounts for the coordinated manifestation of the genetic programme underlying embryonic development and cell differentiation. The 3' untranslated region (3'-UTR) of eukaryotic genes can contain motifs involved in regulation of gene expression at the post-transcriptional level. In the 3'-UTR of dmrt1, a key gene that functions in gonad development and differentiation, an 11-bp protein-binding motif was identified that mediates gonad-specific mRNA localization during embryonic and larval development of fish. Mutations that disrupt the 11-bp motif leading to in vitro protein-binding loss and selective transcript stabilization failure indicate a role for this motif in RNA stabilization through protein binding. The sequence motif was found to be conserved in most of the dmrt1 homologous genes from flies to humans suggesting a widespread conservation of this specific mechanism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping