ZFIN ID: ZDB-PUB-110131-37
Structures of SAS-6 Suggest Its Organization in Centrioles
van Breugel, M., Hirono, M., Andreeva, A., Yanagisawa, H.A., Yamaguchi, S., Nakazawa, Y., Morgner, N., Petrovich, M., Ebong, I.O., Robinson, C.V., Johnson, C.M., Veprintsev, D., and Zuber, B.
Date: 2011
Source: Science (New York, N.Y.)   331(6021): 1196-1199 (Journal)
Registered Authors: Johnson, Christopher
Keywords: none
MeSH Terms:
  • Amino Acid Motifs
  • Animals
  • Cell Cycle Proteins/chemistry
  • Cell Cycle Proteins/metabolism
  • Cell Line, Tumor
  • Centrioles/chemistry*
  • Centrioles/metabolism
  • Centrioles/ultrastructure
  • Centrosome/metabolism
  • Chlamydomonas reinhardtii/chemistry
  • Chlamydomonas reinhardtii/metabolism
  • Chromosomal Proteins, Non-Histone/chemistry*
  • Chromosomal Proteins, Non-Histone/metabolism
  • Crystallography, X-Ray
  • Flagella/metabolism
  • Flagella/ultrastructure
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Models, Molecular
  • Mutant Proteins/chemistry
  • Point Mutation
  • Protein Multimerization
  • Protein Structure, Quaternary
  • Protein Structure, Tertiary
  • Recombinant Proteins/chemistry
  • Recombinant Proteins/metabolism
  • Zebrafish
  • Zebrafish Proteins/chemistry*
  • Zebrafish Proteins/metabolism
PubMed: 21273447 Full text @ Science
Centrioles are cylindrical, nine-fold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the N-terminal domain of SAS-6 from zebrafish and show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are likely essential to the structural organization of cartwheel centers.