Microarray Analysis of XOPS-mCFP Zebrafish Retina Identifies Genes Associated with Rod Photoreceptor Degeneration and Regeneration
- Morris, A.C., Forbes-Osborne, M.A., Pillai, L.S., and Fadool, J.M.
- Investigative ophthalmology & visual science 52(5): 2255-2266 (Journal)
- Registered Authors
- Morris, Ann C.
- MeSH Terms
- Animals, Genetically Modified
- Fluorescent Antibody Technique, Indirect
- Gene Expression Profiling
- Gene Expression Regulation/physiology*
- Green Fluorescent Proteins/genetics*
- In Situ Hybridization
- Microarray Analysis
- Proliferating Cell Nuclear Antigen/metabolism
- Proto-Oncogene Proteins c-myb/metabolism
- RNA, Messenger/genetics
- Recombinant Fusion Proteins/genetics*
- Retinal Degeneration/genetics*
- Retinal Degeneration/metabolism
- Retinal Degeneration/physiopathology
- Retinal Rod Photoreceptor Cells/physiology*
- Reverse Transcriptase Polymerase Chain Reaction
- SOX Transcription Factors/metabolism
- Transcription Factors/metabolism
- Zebrafish Proteins/metabolism
- 21217106 Full text @ Invest. Ophthalmol. Vis. Sci.
Morris, A.C., Forbes-Osborne, M.A., Pillai, L.S., and Fadool, J.M. (2011) Microarray Analysis of XOPS-mCFP Zebrafish Retina Identifies Genes Associated with Rod Photoreceptor Degeneration and Regeneration. Investigative ophthalmology & visual science. 52(5):2255-2266.
PURPOSE. XOPS-mCFP transgenic zebrafish experience a continual cycle of rod photoreceptor development and degeneration throughout life, making them a useful model to investigate the molecular determinants of rod photoreceptor regeneration. The purpose of this study was to compare the gene expression profiles of wild type and XOPS-mCFP retinas in order to identify genes that may contribute to the regeneration of the rods. METHODS. Adult wild type and XOPS-mCFP retinal mRNA was subjected to microarray analysis using the Agilent platform. The Ingenuity Pathway Analysis program was used to identify biologically relevant processes that were significantly represented in the dataset. Expression changes were verified by RT-PCR. Selected genes were further examined during retinal development and in adult retinas by in situ hybridization, immunohistochemistry, and using a transgenic fluorescent reporter line. RESULTS. Over 600 genes displayed significant expression changes in XOPS-mCFP retinas compared to wild type controls. Many of the downregulated genes were associated with phototransduction, whereas upregulated genes were associated with several biological functions, including cell cycle, DNA replication and repair, cell development and cell death. RT-PCR analysis of a subset of these genes confirmed the microarray RESULTS: Three transcription factors (sox11b, insm1a, and c-myb) displaying increased expression in XOPS-mCFP retinas were also expressed throughout retinal development and in the persistently neurogenic ciliary marginal zone. CONCLUSIONS. This study identified numerous gene expression changes in response to rod degeneration in zebrafish, and further suggests a role for the transcriptional regulators sox11b, insm1a, and c-myb in both retinal development and rod photoreceptor regeneration.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes