PUBLICATION
Pharmacological analysis of zebrafish (Danio rerio) scototaxis
- Authors
- Maximino, C., da Silva, A.W., Gouveia, A. Jr, and Herculano, A.M.
- ID
- ZDB-PUB-110119-36
- Date
- 2011
- Source
- Progress in neuro-psychopharmacology & biological psychiatry 35(2): 624-631 (Journal)
- Registered Authors
- Maximino, Caio
- Keywords
- Anxiety, Light/dark preference, Zebrafish
- MeSH Terms
-
- Adaptation, Ocular/drug effects
- Animals
- Anti-Anxiety Agents/pharmacology*
- Antidepressive Agents/pharmacology*
- Anxiety/drug therapy
- Behavior, Animal/drug effects*
- Behavior, Animal/physiology
- Benzodiazepines/pharmacology*
- Caffeine/pharmacology
- Central Nervous System Depressants/pharmacology
- Central Nervous System Stimulants/pharmacology
- Dark Adaptation/drug effects*
- Dose-Response Relationship, Drug
- Ethanol/pharmacology
- Motor Activity/drug effects*
- Motor Activity/physiology
- Zebrafish
- PubMed
- 21237231 Full text @ Prog. Neuropsychopharmacol. Biol. Psychiatry
Citation
Maximino, C., da Silva, A.W., Gouveia, A. Jr, and Herculano, A.M. (2011) Pharmacological analysis of zebrafish (Danio rerio) scototaxis. Progress in neuro-psychopharmacology & biological psychiatry. 35(2):624-631.
Abstract
The scototaxis test has been introduced recently to assess anxiety-like phenotypes in fish, including zebrafish. Parametric analyses suggest that scototaxis represents an approach-avoidance conflict, which hints at anxiety. In this model, white avoidance represents anxiety-like behavior, while the number of shuttling events represents activity. Acute or chronic fluoxetine, buspirone, benzodiazepines, ethanol, caffeine and dizocilpine were assessed the light-dark box (scototaxis) test in zebrafish. Acute fluoxetine treatment did not alter white avoidance, but altered locomotion in the higher dose; chronic treatment (2weeks), on the other hand, produced an anxiolytic effect with no locomotor outcomes. The benzodiazepines produced a hormetic (inverted U-shaped) dose-response profile, with intermediate doses producing anxiolysis and no effect at higher doses; clonazepam, a high-potency benzodiazepine agonist, produced a locomotor impairment at the highest dose. Buspirone produced an anxiolytic profile, without locomotor impairments. Moclobemide did not produce behavioral effects. Ethanol also produced a hormetic profile in white avoidance, with locomotor activation in 0.5% concentration. Caffeine produced an anxiogenic profile, without locomotor effects. These results suggest that the light-dark box is sensitive to anxiolytic and anxiogenic drugs in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping