ZFIN ID: ZDB-PUB-110110-7
Integrated biological responses of zebrafish (Danio rerio) to analyze water quality in regions under anthropogenic influence
Amado, L.L., Rosa, C.E., Castro, M.R., Votto, A.P., Santos, L.C., Marins, L.F., Trindade, G.S., Fraga, D.S., Damé, R.C., Barros, D.M., Geracitano, L.A., Bianchini, A., de la Torre, F.R., and Monserrat, J.M.
Date: 2011
Source: Chemosphere   82(11): 1563-1570 (Journal)
Registered Authors: Barros, Daniela Marti, Marins, Luis Fernando
Keywords: Antioxidant responses, Zebrafish, Long-term memory, Ecotoxicology, Total antioxidant capacity, Reactive oxygen species
MeSH Terms:
  • Animals
  • Biomarkers/metabolism
  • Environmental Monitoring/methods*
  • Fresh Water/chemistry
  • Gills/drug effects
  • Gills/metabolism
  • Glutamate-Cysteine Ligase/metabolism
  • Glutathione/metabolism
  • Hepatocytes/drug effects
  • Hepatocytes/metabolism
  • Reactive Oxygen Species/metabolism
  • Water Pollutants, Chemical/analysis
  • Water Pollutants, Chemical/toxicity*
  • Water Supply/analysis
  • Zebrafish/metabolism
  • Zebrafish/physiology*
PubMed: 21194724 Full text @ Chemosphere
This study analyzed water quality in regions around Patos lagoon (Southern Brazil) that are under anthropogenic pressure. Water samples were collected from five different sites, including one used as a source for human consumption (COR) and others known to be influenced by human activities (IP). Danio rerio (Teleostei, Cyprinidae) organisms were exposed for 24h to these water samples, plus a control group. It was observed that: (1) reactive oxygen species levels were lower in COR and IP than in the control group; (2) glutamate-cysteine ligase (catalytic subunit) expression was higher in COR than in other sites; (3) exposure to all water samples affected long-term memory (LTM) when compared to control group. Thus, some water samples possess the ability to modulate the antioxidant system and to induce a decline in cognitive functions, as measured by LTM. The obtained results indicate that a combination of variables of different organization level (molecular, biochemical and behavioral) can be employed to analyze water quality in impacted regions.