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Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
GOA, HGNC, AgBase and UniProtKB curators
ZFIN Direct Data Submission:
GOA, HGNC, AgBase and UniProtKB curators (2007) Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity. ZFIN Direct Data Submission. . (http://zfin.org).
Method for transferring manual annotations to an entry based on a
curator's judgment of its similarity to a putative ortholog which has
annotations with experimental evidence. Annotations are created when a
curator judges that the sequence of a protein shows high similarity to
another protein that has annotation(s) supported by experimental
evidence (IDA, IGI, IMP, IPI or IEP). Annotations resulting from the
transfer of GO terms display the 'ISS' evidence code and include an
accession for the protein from which the annotation was projected in
the 'with' field (column 8). This field can contain either a UniProtKB
Accession or an IPI (International Protein Index) identifier. Only
annotations with an experimental evidence code and which do not have
the 'NOT' qualifier are transferred. Orthologs/homologs are chosen
following a protein MPsrch (http://www.ebi.ac.uk/MPsrch) or BLAST,
where the aligned sequences show a high degree of similarity over
their entire lengths, making it reasonable to infer that the two
proteins have a common function. It must be emphasized that curators
must check each alignment and use their experience to assess whether
similarity is considered to be strong enough to project annotations.
While there is no fixed cut-off point in percentage sequence
similarity, generally proteins which have greater than 60% identity
that covers greater than 80% of the length of both proteins are
examined further. For mammalian proteins this cut-off tends to be
higher, with an average of 80% identity over 90% of the length of both
proteins. Additional tools, such as the HCOP orthology tool (http://www.gene.ucl.ac.uk/cgi-bin/nomenclature/hcop.pl
), are used when possible. Strict orthologs are desirable but not
essential. When there is evidence of paralogs, annotations are
transferred only to the most similar protein in each species. Further
detailed information on this procedure, including how ISS annotations
are made to protein isoforms, can be found at: http://www.ebi.ac.uk/GOA/ISS_method.html.