PUBLICATION
Proteome profile of zebrafish caudal fin based on one-dimensional gel electrophoresis LCMS/MS and two-dimensional gel electrophoresis MALDI MS/MS analysis
- Authors
- Singh, S.K., Meena Lakshmi, M.G., Saxena, S., Swamy, C.V., and Idris, M.M.
- ID
- ZDB-PUB-101209-17
- Date
- 2011
- Source
- Journal of separation science 34(2): 225-232 (Journal)
- Registered Authors
- Idris, Mohammed, Singh, Sachin
- Keywords
- Caudal fin, Fin proteome, Network pathway, Zebrafish
- MeSH Terms
-
- Animal Fins/chemistry*
- Animal Fins/metabolism
- Animals
- Electrophoresis, Gel, Two-Dimensional/methods*
- Electrophoresis, Polyacrylamide Gel/methods
- Female
- Fish Proteins/chemistry
- Fish Proteins/metabolism
- Male
- Molecular Sequence Data
- Proteome/chemistry*
- Proteome/metabolism
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
- Tandem Mass Spectrometry/methods
- Zebrafish/metabolism*
- PubMed
- 21246729 Full text @ J. Sep. Sci.
Citation
Singh, S.K., Meena Lakshmi, M.G., Saxena, S., Swamy, C.V., and Idris, M.M. (2011) Proteome profile of zebrafish caudal fin based on one-dimensional gel electrophoresis LCMS/MS and two-dimensional gel electrophoresis MALDI MS/MS analysis. Journal of separation science. 34(2):225-232.
Abstract
Zebrafish (Danio rerio) is the widely used vertebrate model animal for understanding the complexity of development and disease process. Zebrafish has been also extensively used in understanding the mechanism of regeneration for its extensive capability of regenerating fins and other tissues. We have analyzed the proteome profile of zebrafish caudal fin in its native state based on one-dimensional gel electrophoresis LCMS/MS and two-dimensional gel electrophoresis MS/MS analyses. A total of 417 proteins were identified as zebrafish fin tissue specific, which includes 397 proteins identified based on one-dimensional gel electrophoresis LCMS/MS analysis and 101 proteins identified based on two-dimensional gel electrophoresis MALDI MS/MS. The proteins mapped to the zebrafish fin tissue were shown to be involved in various biological activities related to development, apoptosis, signaling and metabolic process. Focal adhesion, regulation of actin cytoskeleton, cancer-related pathways, mitogen-activated protein kinase signaling, antigen processing and presentation, and proteasome are some of the important pathways associated with the identified proteome data set of the zebrafish fin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping