PUBLICATION
The Fz-Dsh Planar Cell Polarity Pathway Induces Oriented Cell Division via Mud/NuMA in Drosophila and Zebrafish
- Authors
- Segalen, M., Johnston, C.A., Martin, C.A., Dumortier, J.G., Prehoda, K.E., David, N.B., Doe, C.Q., and Bellaiche, Y.
- ID
- ZDB-PUB-101122-5
- Date
- 2010
- Source
- Developmental Cell 19(5): 740-752 (Journal)
- Registered Authors
- David, Nicholas
- Keywords
- none
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing/genetics
- Adaptor Proteins, Signal Transducing/metabolism*
- Animals
- Cell Division/physiology*
- Cell Line
- Cell Lineage
- Cell Polarity/physiology*
- Drosophila Proteins/genetics
- Drosophila Proteins/metabolism*
- Drosophila melanogaster*/cytology
- Drosophila melanogaster*/physiology
- Dyneins/metabolism
- Embryo, Nonmammalian/anatomy & histology
- Embryo, Nonmammalian/physiology
- Frizzled Receptors/genetics
- Frizzled Receptors/metabolism*
- Gastrulation
- Guanine Nucleotide Dissociation Inhibitors/genetics
- Guanine Nucleotide Dissociation Inhibitors/metabolism
- Humans
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Nuclear Matrix-Associated Proteins/genetics
- Nuclear Matrix-Associated Proteins/metabolism*
- Phosphoproteins/genetics
- Phosphoproteins/metabolism*
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Signal Transduction/physiology
- Spindle Apparatus/metabolism
- Zebrafish*/anatomy & histology
- Zebrafish*/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 21074723 Full text @ Dev. Cell
Citation
Segalen, M., Johnston, C.A., Martin, C.A., Dumortier, J.G., Prehoda, K.E., David, N.B., Doe, C.Q., and Bellaiche, Y. (2010) The Fz-Dsh Planar Cell Polarity Pathway Induces Oriented Cell Division via Mud/NuMA in Drosophila and Zebrafish. Developmental Cell. 19(5):740-752.
Abstract
The Frizzled receptor and Dishevelled effector regulate mitotic spindle orientation in both vertebrates and invertebrates, but how Dishevelled orients the mitotic spindle is unknown. Using the Drosophila S2 cell "induced polarity" system, we find that Dishevelled cortical polarity is sufficient to orient the spindle and that Dishevelled's DEP domain mediates this function. This domain binds a C-terminal domain of Mud (the Drosophila NuMA ortholog), and Mud is required for Dishevelled-mediated spindle orientation. In Drosophila, Frizzled-Dishevelled planar cell polarity (PCP) orients the sensory organ precursor (pI) spindle along the anterior-posterior axis. We show that Dishevelled and Mud colocalize at the posterior cortex of pI, Mud localization at the posterior cortex requires Dsh, and Mud loss-of-function randomizes spindle orientation. During zebrafish gastrulation, the Wnt11-Frizzled-Dishevelled PCP pathway orients spindles along the animal-vegetal axis, and reducing NuMA levels disrupts spindle orientation. Overall, we describe a Frizzled-Dishevelled-NuMA pathway that orients division from Drosophila to vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping