PUBLICATION
UV-induced fin damage in zebrafish as a system for evaluating the chemopreventive potential of broccoli and cauliflower extracts
- Authors
- Chen, Y.H., Wen, C.C., Lin, C.Y., Chou, C.Y., Yang, Z.S., and Wang, Y.H.
- ID
- ZDB-PUB-101115-17
- Date
- 2011
- Source
- Toxicology mechanisms and methods 21(1): 63-69 (Journal)
- Registered Authors
- Chen, Yau-Hung
- Keywords
- Broccoli, cauliflower, fin, skin cancer, UVB, zebra fish
- MeSH Terms
-
- Animal Fins/radiation effects*
- Animals
- Brassica/chemistry*
- Larva/drug effects
- Plant Extracts/chemistry
- Plant Extracts/pharmacology*
- Ultraviolet Rays*
- Zebrafish/embryology
- PubMed
- 21067310 Full text @ Toxicol. Mech. Methods
Citation
Chen, Y.H., Wen, C.C., Lin, C.Y., Chou, C.Y., Yang, Z.S., and Wang, Y.H. (2011) UV-induced fin damage in zebrafish as a system for evaluating the chemopreventive potential of broccoli and cauliflower extracts. Toxicology mechanisms and methods. 21(1):63-69.
Abstract
This study applied broccoli and cauliflower extracts (whole, floret, and stem) to zebrafish larvae in parallel to receive 100 mJ/cm(2) of UVB six times, and recorded their fin malformation phenotypes. Chemopreventive effects of each group, including UVB, whole-, floret-, and stem-extracts of broccoli and cauliflower on fin development were evaluated using Kaplan-Meier analysis, log-rank test, and Cox proportional hazards regression. Results showed that (1) zebrafish fins in the UVB + whole broccoli extract group are 6.20~9.32-times more likely to return to normal fins than ones in the UVB only group, but fins in the UVB + whole cauliflower extract group are only 5.13~11.10-times more likely to recover, indicated that whole broccoli and cauliflower extract had similar chemopreventive ability on fin development; and (2) the broccoli stem has the highest antioxidant capacity among other groups. In conclusion, zebrafish can be used as a system for evaluating the efficacy of other UVB protective compounds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping